Invitrogen™

eBioscience™ DRAQ5™

Catalog number: 65-0880-96
Invitrogen™

eBioscience™ DRAQ5™

Catalog number: 65-0880-96
Catalog Number
65-0880-96
Unit Size
200 µL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
50 µL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
65-0880-96200 µL
-
65-0880-9250 µL
-
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DRAQ5™ is an anthraquinone dye with high affinity for double-stranded DNA. It is a membrane-permeable dye that can label live or fixed/dead cells. In flow cytometry, this dye can be used to distinguish nucleated and non-nucleated cells. DRAQ5™ can also be used to report nuclear DNA content for ploidy and cell cycle analysis because it binds DNA stoichiometrically. In fluorescent microscopy, it can be used as a nuclear counterstain.

DRAQ5™ can be excited by wavelengths from 488 to 647 nm. For microscopy/imaging, 633 or 647 nm sources for excitation are recommended. For flow cytometry, with excitation at 488 nm, this dye can be detected with filter sets designed for PerCP-eFluor™ 710 or PerCP-Cy5.5 (685LP dichroic mirror and 710/50 band pass); with excitation at 633 nm, it can be detected with filter sets designed for APC (660/20 band pass). For cell cycle/DNA analysis applications, a higher wavelength filter is recommended, such as a 710LP or a filter set designed for PE-Cy7 (735LP dichroic mirror and 780/60 band pass), to optimize the CV for the G1 and G2/M peaks. Please make sure that your instrument is capable of detecting this dye.

Because of its broad excitation and emission properties, DRAQ5™ is not recommended in combination with other far-red fluorochromes excited by the 488 or 633 nm laser lines, including PE-Cy7, PerCP-eFluor™ 710, PerCP-Cy5.5, APC, Alexa Fluor™ 647, Alexa Fluor™ 700, APC-eFluor™ 780 and equivalents (APC-Alexa Fluor™ 750 and APC-Cy7).

Reported Application
Cell Labeling, Immunocytochemistry, Flow Cytometric Analysis, Microscopy

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color
Red
Detection Method
Fluorescent
For Use With (Application)
Immunohistochemistry (IHC), Flow Cytometry
For Use With (Equipment)
Fluorescence Microscope
Product Type
Dye
Emission
488-647⁄633-710
Product Line
eBioscience™
Quantity
200μL

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