Invitrogen™

Dynabeads™ In Vitro Transcription and RNA Purification Kit

Catalog number: 65036D
Invitrogen™

Dynabeads™ In Vitro Transcription and RNA Purification Kit

Catalog number: 65036D
Catalog Number
65036D
Unit Size
10 mL
Price (USD)
Price: 3,070.00
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
2 mL
Price (USD)
Price: 845.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
65036D10 mL
Price: 3,070.00
Your price:
-
65034D2 mL
Price: 845.00
Your price:
-
Product Overview
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The Dynabeads In Vitro Transcription and RNA Purification Kit offers an innovative technology for simple, flexible, and automatable mRNA production and is particularly suitable where high quality and scalability are required, such as in the discovery phase of RNA-based vaccines and therapeutics development. This automation-ready solution can help speed up the screening of hundreds of constructs that may be required to identify a final drug candidate, in scalable quantities to match your requirements.

Features of the Dynabeads In Vitro Transcription and RNA Purification Kit include:
Flexibility—choose DNA template for IVT with flexibility of design and amplification
Efficiency—reuse DNA template and beads (at least six times) to help reduce scost and eliminate/minimize plasmid prep hands-on time and risk of bacterial host DNA transfer
Scalability—swap between manual and automated workflows to match required scale (micrograms to grams of mRNA) and throughput
Quality—help ensure a safe and flexible supply of magnetic beads to navigate from RNA-based drug discovery research to commercial manufacturing with counterpart solutions (available via the OEM commercial supply), without incurring extra time or costs from switching technology or raw material

Overview of the workflow
A biotinylated template is made by PCR amplification of the target sequence in a plasmid construct or in a synthetic DNA construct using a biotinylated forward primer and a non-biotinylated reverse primer (figure below). As an alternative to PCR amplification, a linearized plasmid can be biotinylated by a fill-in reaction of biotin-dUTP in the 5' overhang sequence, provided the correct plasmid design. The biotinylated template is then immobilized directly to the included Dynabeads Streptavidin for In Vitro Transcription beads without the need for prior purification. The bead-bound template is used directly in the IVT, then removed from the synthesized mRNA by magnetic separation. The template can be reused (at least six times) in consecutive IVT reactions (figure below).

Solid-phase IVT is compatible with commonly used reagents such as the MEGAscript T7 Transcription Kit, mMESSAGE mMACHINE T7 Transcription Kit, and mMESSAGE mMACHINE T7 ULTRA Transcription Kit, based on your needs and strategies for capping and polyadenylation.

Crude RNA from the IVT step is mixed with the included Dynabeads Carboxylic Acid for RNA Purification and the included Dynabeads RNA Binding Buffer is added. The RNA binds to the bead surface and the remaining components of the reaction mixture are removed by applying a magnet (see thermofisher.com/magnets) and discarding the supernatant (figure below).

Automation-ready for use with KingFisher purification systems
The 1-μm magnetic beads are ideal for high-throughput screening. The process can be automated using liquid handlers or any of our KingFisher sample purification systems. Automation with a KingFisher instrument enables IVT template immobilization, IVT reaction, and purification of 96 samples in less than four hours, and up to 5 mg mRNA yield per well. The scripts for KingFisher Flex and KingFisher Apex automated purification workflows are available from our website.

Commercial supply
To learn more about counterpart products and support documentation available for GMP-compliant manufacturing of mRNA vaccines and therapeutics, visit: thermofisher.com/dynabeadsmrna.

Specifications

Description
Large Combo Kit
Isolation Technology
Magnetic Bead
Purification Time
Variable
For Use With (Equipment)
KingFisher™ Sample Purification System, DynaMag™ magnets
For Use With (Application)
In Vitro Transcription, Synthetic RNA, Crude RNA Purification (from IVT Reaction Mix)
Product Line
Dynabeads™
RNase
RNase-free (No detectable activity level)
Shelf Life
18 Months (from date of manufacture)
Shipping Condition
Ambient Temperature
Sterility
Non-sterile

Contents & Storage

• 1 unit of Dynabeads Streptavidin for In Vitro Transcription (Cat. No. 65006D). Store at 2–8°C.
• 1 unit of Dynabeads Carboxylic Acid for RNA Purification (Cat. No. 65021D). Store at 2–8°C.
• 1 unit of Dynabeads RNA Binding Buffer (Cat. No. 65042D). Store at 2–8°C. Protect from light exposure during storage.

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