Slide-A-Lyzer Dialysis Cassettes (3.5K MWCO) facilitate simple and effective removal of salts and small contaminants from proteins and other macromolecules larger than 3,500 Daltons. They can be used for a wide range of applications including low-molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration.

Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips, or caps are needed to seal the units and prevent leaking and sample loss during the dialysis procedure. Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

Features of Slide-A-Lyzer Dialysis Cassettes:
• Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
• Fast dialysis—flat cassette chamber with two membranes provides high surface area-to-volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
• High recovery—rectangular cassette design maximizes recovery of entire sample volume from any one of the four corner injection ports
• Four cassette sizes—select the cassette that best suits your sample volume (0.5 to 30 mL)
• Color-coded—pink cassette frame specifies cassettes with the 3,500 MW pore size membrane

Membrane specifications:
• Composition: regenerated cellulose synthesized by the viscose method
• Thickness: 0.9 to 1.2 mil (22.5 to 30 μm)
• Hydration time required before use: 2 minutes
• Glycerol content: none
• Sulfur content: 0.1–0.15%
• Heavy metals content: trace

Volume ranges:
• 0.5 mL = 0.1 to 0.5 mL
• 3 mL = 0.5 to 3 mL
• 12 mL = 3 to 12 mL
• 30 mL = 12 to 30 mL