Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices, 7K MWCO are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules >7kDa in volumes from 10 to 100 µL.
Features of Slide-A-Lyzer MINI Dialysis Device, 7K MWCO, 0.1 mL:
• Flexible format - enables user to dialyze a single sample (tube) or multiple samples (25 array) • Excellent sample recoveries - low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems • One-step protocol - does not require laborious procedures or expensive equipment • 100% leak-tested - innovative design does not permit 'wicking' that can occur in home-made devices • Time savings - performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation
Slide-A-Lyzer MINI Dialysis Devices allow easy sample addition and removal using a standard laboratory pipette and can be used for single or arrays of samples. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.
Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.
The 0.1 mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro centrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1 mL MINI Device, 100 µL of pH 2.8 glycine buffer can be equilibrated with 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.
As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.