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TNF (TNFA) Recombinant Canine Protein

Catalog number: 70003DNAE5

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Tumor Necrosis Factor (TNF), recombinant canine protein is supplied in frozen format. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications

This recombinant protein was expressed from a DNA sequence encoding the soluble form of canine TNFalpha (NP_001003244.4) (Val 77-Leu 233) fused to an initial Met at the N-terminus.

Activity: Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 is typically 0.2-1.2 ng/ml.

Formulation: 50 mM Tris, 500 mM NaCl, 20% glycerol, pH 8.0.

Reconstitution: No reconstitution needed. Store at -80°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.


Research Category: Apoptosis, Cell Cycle & Proliferation, Inflammation, Oncology
Expression: E. coli
Form: Frozen
Gene Aliases: TNFA, Ctnf
Gene Symbol: TNF
Molecular Weight: 17.3 kDa
Product Line: Sino Biological®
Product Size: 5 µg
Protein Name: tumor necrosis factor
Protein Subtype: TNF (Tumor Necrosis Factor)
Protein Tag or Fusion: Untagged
Purity: ≥ 90% by SDS-PAGE
Species: Dog⁄Canine
Validated Application: Assay Standard

Contents & storage

Store at -80 °C.


Manuals & protocols

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