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EGF Recombinant Canine Protein. His Tag

Catalog number: 70013D07E5

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Epidermal growth factor (EGF), recombinant canine protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. In general, recombinant proteins can be used as protein standard and in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form (Asn 973-Arg 1024) of canine EGF (Q9BEA0) fused to a polyhistidine tag at the N-terminus.

Activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1-0.5 ng/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.


Form: Lyophilized
Purity: ≥ 95% by SDS-PAGE
Gene ID: 403657
Species: Dog⁄Canine
Expression: E. coli
Gene Symbol: EGF
Gene Aliases: CEGF
Product Size: 5 µg
Protein Form: Recombinant, Ligand
Protein Family: Growth Factors & Receptors
Protein Subtype: EGF (Epidermal Growth Factor)
Molecular Weight: 8.3 kDa
Research Category: Cell Cycle & Proliferation, Other Research
Protein Tag or Fusion: His Tag
Validated Application: Assay Standard

Contents & storage

Store at -20 to -70 °C


Manuals & protocols

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