Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Features
This primer-sequestration technique effectively blocks DNA synthesis from mis-priming events at lower temperatures. Following the initial denaturation step during PCR, the protein is inactivated, and the primers are free to participate in the amplification reaction.
HotStart-IT Binding Protein performs well in many standard PCR reaction buffers and has been designed for PCR applications that demand high specificity and sensitivity. PCR with Taq DNA Polymerase and HotStart-IT Binding Protein shifts production of primer-dimers to a specific target of 306 bp from 1 ng of human genomic DNA relative to Taq DNA Polymerase alone.
Application
Notes