HotStart-IT Binding Protein
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Thermo Scientific™

HotStart-IT Binding Protein

HotStart-IT Binding Protein is the active component in a novel hot start technology called primer sequestration.
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Catalog number 71194400UG
also known as 71194 400 UG
Price (USD)/ Each
161.00
-
Add to cart
Price (USD)/ Each
161.00
Add to cart
HotStart-IT Binding Protein
Catalog number71194400UG
Price (USD)/ Each
161.00
-
Add to cart
HotStart-IT Binding Protein is the active component in the novel hot start technology known as primer sequestration. In primer sequestration, hot start PCR methods reduce or eliminate non-specific primer-extension products formed at lower temperatures during assembly of PCR reactions. At these less stringent annealing temperatures, primers may bind non-specifically, which often leads to unwanted amplification products and primer-dimers. To resolve this problem, we have produced a high-quality DNA binding protein that is especially useful at sequestering primers at lower temperatures, making them unavailable for use by a polymerase (see figure).

Features

  • Room temperature reaction setup
  • Minimizes amplification of non-specific products and primer-dimers (see figure)
  • Ideal for complex templates and multiplex reactions
  • No extensive heating step required
  • Free from detectable non-specific nucleases
  • Technology is portable to a polymerase of choice

This primer-sequestration technique effectively blocks DNA synthesis from mis-priming events at lower temperatures. Following the initial denaturation step during PCR, the protein is inactivated, and the primers are free to participate in the amplification reaction.

HotStart-IT Binding Protein performs well in many standard PCR reaction buffers and has been designed for PCR applications that demand high specificity and sensitivity. PCR with Taq DNA Polymerase and HotStart-IT Binding Protein shifts production of primer-dimers to a specific target of 306 bp from 1 ng of human genomic DNA relative to Taq DNA Polymerase alone.

Application

  • Hot-Start PCR amplification

Notes

  • One microgram of HotStart-IT Binding Protein sequesters about 5 pmol of primers.
  • HotStart-IT Binding Protein is sourced from recombinant protein expressed in E. coli.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Hot-start PCR
Shipping ConditionDry Ice
Compatible Buffer1X PCR Reaction Buffer
Quantity400 μg (200 x 25 μL reactions)
Product TypeHotStart-IT Binding Protein
Unit SizeEach
Contents & Storage
• HotStart-IT Binding Protein, 400 μg

Store at -20°C.