ExoSAP-IT™ Express PCR Product Cleanup Reagent

Catalog number: 75001.200.UL

Applied Biosystems™  Related applications: PCR

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Description

In only 5 minutes, ExoSAP-IT™ Express PCR Product Cleanup Reagent removes excess primers and unincorporated nucleotides from a PCR reaction. This enzymatic cleanup method offers precision, increased yield, and a faster turnaround time compared to alternative purification methods, such as spin columns or beads.

• Remove unincorporated primers and nucleotides in about 5 minutes
• Prepare DNA sequencing samples with one pipetting step
• Conserve precious PCR products with up to 100% recovery, regardless of amplicon length
• Minimize spin column or bead cleanup

ExoSAP-IT Express PCR Product Cleanup Reagent consists of two hydrolytic enzymes: a novel exonuclease I and shrimp alkaline phosphatase (SAP), in a specially formulated buffer. The reaction setup is complete with one pipetting step, which is followed by two short incubations. The first incubation hydrolyzes excess primer and dephosphorylates nucleotides. The second, high-temperature incubation completely and irreversibly inactivates the enzymes in about one minute. Adding ExoSAP-IT Express reagent directly to the PCR product eliminates transfer steps to tubes, wells, or columns, conserves PCR amplicons, helping reduce the chance of cross contamination; no further processing is needed. Samples are ready for DNA sequencing or SNP analysis.

Specifications

Research Category: Genetic Diseases
Product Line: ExoSAP-IT™
Product Size: 100 reactions
Protein Form: Recombinant
Shipping Condition: Dry Ice
Validated Application: PCR Purification

Contents & storage

Store at -5 to -30°C.

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