Thermo Scientific™

Yeast beta-Galactosidase Assay Kit

Catalog number: 75768
Thermo Scientific™

Yeast beta-Galactosidase Assay Kit

Catalog number: 75768
Catalog Number
75768
Unit Size
75 mL
Price (USD)
Price: 248.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
7576875 mL
Price: 248.00
Your Price:
-
Product Overview
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Thermo Scientific Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in-vivo using two-hybrid systems.

Features of Yeast beta-Galactosidase Assay Kit:

• Efficient lysis of yeast cells and a colorimetric detection system
• Quantitative or qualitative assay for yeast colonies grown on solid media or in suspension
• Colorimetric assay easily measured in a plate reader or spectrophotometer (420nm)
• Adaptable to 96-well microplates or tube assay formats
• Allows cells obtained from solid media or liquid culture to be assayed directly with no harvesting or washing steps.• Perform homogeneous screening assays or measure activity secondarily after cell lysis
• Also compatible with prepared lysates from other species such as bacteria

Yeast β-Galactosidase Assay Kit allows qualitative or quantitative determination of β-galactosidase activity in solution directly from colonies growing on solid medium. A portion of the colony is suspended in a mixture of Thermo Scientific Y-PER Yeast Protein Extraction Reagent and β-Galactosidase Assay Buffer. After a brief incubation period, the solution turns yellow from the hydrolysis of o-nitrophenyl-β-D-galactopyranoside (ONPG) to o-nitrophenol (ONP) and galactose in a mildly alkaline solution. The assay becomes quantitative if calibrated to cell number by measuring the optical density at 660nm.

The gene encoding β-galactosidase (lacZ) of E. coli has been widely used as a reporter gene in many different prokaryotic and eukaryotic organisms. β-Galactosidase activity is also commonly used as an indicator of protein-protein interactions in-vivo using two-hybrid systems. The interaction strength is verified or quantitated using a β-galactosidase activity assay.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Reporter Enzyme
For Use With (Application)
Absorbance
For Use With (Equipment)
Microplate Reader
Product Type
Yeast beta-Galactosidase Assay Kit
Dye Type
Enzyme Labeled
Format
96-well Plate
Product Line
Pierce™
Substrate
ONPG
Substrate Type
Beta-Gal Substrate
Sufficient For
250 colonies or 250 culture samples (100 µL each)
Target
Beta-Galactosidase

Contents & Storage

Sufficient For: 250 colonies or 250 culture samples (100 μL each)
  • Y-PER Yeast Protein Extraction Reagent, 25 mL (store at room temperature)
  • 2X beta-Galactosidase Assay Buffer, 25 mL (store at -20°C)
  • 1M Sodium Carbonate Stop Solution, 25 mL (store at room temperature)