The PrioCHECK® PRV gB ELISA is a blocking ELISA that detects antibodies against the Pseudorabies virus (PRV) glycoprotein B in pig serum samples. The samples are incubated in a microtiter plate coated with an inactivated PRV antigen. After washing, the plates are incubated with a monoclonal antibody (mAb) directed against an epitope located on glycoprotein B (gB) of the PRV. This mAb is pre-labeled with an enzyme that generates a color signal. The signal is measured and when no color is formed the sample contains anti-PRV antibodies that competed for the PRV antigen. With this result, the sample is positive for PRV.