Thermo Scientific™

PrioCHECK™ CSFV Erns Antibody ELISA Kit, strip

Catalog number:  7610370

Related applications:

Animal Health

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Description

The PrioCHECK® CSFV Erns ELISA is a blocking ELISA specifically designed to detect pigs infected with the Classical Swine Fever Virus when vaccinated with the CSFV E2-subunit vaccine. The test is based on the blocking, by specific serum antibodies, of the binding between the Erns-specific monoclonal antibodies (mAbs) and the Erns-protein. The PrioCHECK® CSFV Erns ELISA should only be used as a discriminating serological test in pig populations vaccinated with an E2-subunit vaccine. The test has been developed for detection in herds by testing an adequate number of randomly selected animals from each herd.

The PrioCHECK® CSFV Erns ELISA uses two different mAbs directed against different epitopes of the Erns protein of CSFV. Antibodies in the sample are incubated with the CSFV Erns antigen. The antibody-antigen complex is then allowed to bind to an anti-CSFV mAb coated to a plate. A second mAb labeled with an enzyme that generates a color signal (the detection antibody) is then added. The signal is measured and when no color is formed, antibodies in the sample have competed for the available viral protein. With this result, the sample is positive for CSFV.
For Veterinary Use Only.

Specifications

Gene ID (Entrez): Non-Gene
Protein Family: Other Proteins
Assay Sensitivity: See product documentation
Gene Symbol: Non-Gene
Target Kit Named: CSFV Erns Antibody
Target Species Primary: Vet Disease Organism
Target Species Named: Porcine
Product Line: PrioCHECK®, Prionics™
Immunoassay Kit Format: Various Other
Target Species Validated: Porcine
Immunoassay Kit Grade: Strip
Target Organism Class: Classical Swine Fever Virus (CSFV)

Contents & storage

5 plates