ExoSAP-IT™ PCR Product Cleanup Reagent is used for enzymatic cleanup of amplified PCR product. It hydrolyzes excess primers and nucleotides in a single step. ExoSAP-IT-purified samples are ready for use in downstream applications such as DNA sequencing or single nucleotide polymorphism (SNP) analysis.
• Remove excess primers and unincorporated nucleotides enzymatically • Prepare DNA sequencing samples with one pipetting step • Conserve precious PCR products with up to 100% recovery • Minimize column cleanup and sample loss
The ExoSAP-IT one-step solution provides a simple way to enzymatically purify a PCR reaction. The reaction setup is complete with one pipetting step, which is followed by two incubations. The first incubation digests excess primer and dephosphorylates nucleotides. The second, high-temperature incubation inactivates the enzymes.
Enzymatic cleanup is a very efficient method for PCR purification. Adding ExoSAP-IT reagent directly to the PCR product eliminates transfer steps to tubes, wells, or columns and conserves PCR amplicons, helping reduce the chance of cross contamination; no further processing needed.
After enzymatic cleanup with ExoSAP-IT reagent, primers and dNTPs will no longer interfere with DNA sequencing or SNP analysis.
ExoSAP-IT™ PCR Product Clean-up Reagent, 100 rxns, Recombinant, Dry Ice, DNA Sequencing or Single Nucleotide Polymorphism (SNP) Analysis, Use with PCR Purification, Store at –5 to °30°C
For Use With (Application)
No. of Reactions
Contents & Storage
Store at -5 to -30°C.
Fig. 2, ExoSAP-IT treatment of PCR products with no sample loss.
Fig. 3, Summary of ExoSAP-IT PCR Product Treatment
Fig. 1, Summary of ExoSAP-IT PCR Product Treatment