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ExoSAP-IT™ PCR Product Cleanup ReagentExoSAP-IT™ PCR Product Cleanup Reagent is used for enzymatic cleanup of amplified PCR product. It hydrolyzes excess primers and nucleotides Read more
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| Catalog Number | Description | For Use With (Application) | Concentration | Quantity |
|---|---|---|---|---|
| 78201.1.ML | ExoSAP-IT™ PCR Product Clean-up Reagent, 500 rxns, Recombinant, Dry Ice, DNA Sequencing or Single Nucleotide Polymorphism (SNP) Analysis, Use with PCR Purification, Store at –5 to °30°C | PCR Purification | 500 rxns | 1 mL |
| 78205.10.ML | ExoSAP-IT™ PCR Product Clean-up Reagent, 5000 rxns, Recombinant, Dry Ice, DNA Sequencing or Single Nucleotide Polymorphism (SNP) Analysis, Use with PCR Purification, Store at –5 to °30°C | PCR Purification | 5000 rxns | 10 mL |
| 78202.4X.1.ML | ExoSAP-IT™ PCR Product Clean-up Reagent, 2000 rxns, Recombinant, Dry Ice, DNA Sequencing or Single Nucleotide Polymorphism (SNP) Analysis, Use with PCR Purification, Store at –5 to °30°C | PCR Purification | 2000 rxns | 4 x 1 mL |
| 78200.200.UL | ExoSAP-IT™ PCR Product Clean-up Reagent, 100 rxns, Recombinant, Dry Ice, DNA Sequencing or Single Nucleotide Polymorphism (SNP) Analysis, Use with PCR Purification, Store at –5 to °30°C | PCR Purification | 100 rxns | 200 μL |
Catalog number 78201.1.ML
Price (USD)/ 500 reactions
542.65
Online exclusive
576.00 Save 33.35 (6%)
-
Description:
ExoSAP-IT™ PCR Product Clean-up Reagent, 500 rxns, Recombinant, Dry Ice, DNA Sequencing or Single Nucleotide Polymorphism (SNP) Analysis, Use with PCR Purification, Store at –5 to °30°C
For Use With (Application):
PCR Purification
Concentration:
500 rxns
Quantity:
1 mL
Price (USD)/ 500 reactions
542.65
Online exclusive
576.00 Save 33.35 (6%)
ExoSAP-IT™ PCR Product Cleanup Reagent
Catalog number78201.1.ML
Price (USD)/ 500 reactions
542.65
Online exclusive
576.00 Save 33.35 (6%)
-
ExoSAP-IT™ PCR Product Cleanup Reagent is used for enzymatic cleanup of amplified PCR product. It hydrolyzes excess primers and nucleotides in a single step. ExoSAP-IT-purified samples are ready for use in downstream applications such as DNA sequencing or single nucleotide polymorphism (SNP) analysis.
• Remove excess primers and unincorporated nucleotides enzymatically
• Prepare DNA sequencing samples with one pipetting step
• Conserve precious PCR products with up to 100% recovery
• Minimize column cleanup and sample loss
The ExoSAP-IT one-step solution provides a simple way to enzymatically purify a PCR reaction. The reaction setup is complete with one pipetting step, which is followed by two incubations. The first incubation digests excess primer and dephosphorylates nucleotides. The second, high-temperature incubation inactivates the enzymes.
Enzymatic cleanup is a very efficient method for PCR purification. Adding ExoSAP-IT reagent directly to the PCR product eliminates transfer steps to tubes, wells, or columns and conserves PCR amplicons, helping reduce the chance of cross contamination; no further processing needed.
After enzymatic cleanup with ExoSAP-IT reagent, primers and dNTPs will no longer interfere with DNA sequencing or SNP analysis.
• Remove excess primers and unincorporated nucleotides enzymatically
• Prepare DNA sequencing samples with one pipetting step
• Conserve precious PCR products with up to 100% recovery
• Minimize column cleanup and sample loss
The ExoSAP-IT one-step solution provides a simple way to enzymatically purify a PCR reaction. The reaction setup is complete with one pipetting step, which is followed by two incubations. The first incubation digests excess primer and dephosphorylates nucleotides. The second, high-temperature incubation inactivates the enzymes.
Enzymatic cleanup is a very efficient method for PCR purification. Adding ExoSAP-IT reagent directly to the PCR product eliminates transfer steps to tubes, wells, or columns and conserves PCR amplicons, helping reduce the chance of cross contamination; no further processing needed.
After enzymatic cleanup with ExoSAP-IT reagent, primers and dNTPs will no longer interfere with DNA sequencing or SNP analysis.
Specifications
Concentration500 rxns
DescriptionExoSAP-IT™ PCR Product Clean-up Reagent, 500 rxns, Recombinant, Dry Ice, DNA Sequencing or Single Nucleotide Polymorphism (SNP) Analysis, Use with PCR Purification, Store at –5 to °30°C
Sample TypePCR Products
For Use With (Application)PCR Purification
No. of Reactions500
Product LineExoSAP-IT™
Protein FormRecombinant
Quantity1 mL
Research CategoryGenetic Diseases
Shipping ConditionDry Ice
Unit Size500 reactions
Contents & Storage
Store at -5 to -30°C.