ExoSAP-IT™ PCR Product Cleanup Reagent

Catalog number: 78201.1.ML

Applied Biosystems™  Related applications: PCR

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Description

ExoSAP-IT™ PCR Product Cleanup Reagent is used for enzymatic cleanup of amplified PCR product. It hydrolyzes excess primers and nucleotides in a single step. ExoSAP-IT-purified samples are ready for use in downstream applications such as DNA sequencing or single nucleotide polymorphism (SNP) analysis.

• Remove excess primers and unincorporated nucleotides enzymatically
• Prepare DNA sequencing samples with one pipetting step
• Conserve precious PCR products with up to 100% recovery
• Minimize column cleanup and sample loss

The ExoSAP-IT one-step solution provides a simple way to enzymatically purify a PCR reaction. The reaction setup is complete with one pipetting step, which is followed by two incubations. The first incubation digests excess primer and dephosphorylates nucleotides. The second, high-temperature incubation inactivates the enzymes.

Enzymatic cleanup is a very efficient method for PCR purification. Adding ExoSAP-IT reagent directly to the PCR product eliminates transfer steps to tubes, wells, or columns and conserves PCR amplicons, helping reduce the chance of cross contamination; no further processing needed.

After enzymatic cleanup with ExoSAP-IT reagent, primers and dNTPs will no longer interfere with DNA sequencing or SNP analysis.

Specifications

Research Category: Genetic Diseases
Product Line: ExoSAP-IT™
Product Size: 500 reactions
Protein Form: Recombinant
Shipping Condition: Dry Ice
Validated Application: PCR Purification

Contents & storage

Store at -5 to -30°C.

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