Applied Biosystems™

ExoSAP-IT™ PCR Product Cleanup Reagent

Catalog number:  78201.1.ML

Related applications:

PCR

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Description

ExoSAP-IT™ PCR Product Cleanup Reagent is used for enzymatic cleanup of amplified PCR product. It hydrolyzes excess primers and nucleotides in a single step. ExoSAP-IT-purified samples are ready for use in downstream applications such as DNA sequencing or single nucleotide polymorphism (SNP) analysis.

• Remove excess primers and unincorporated nucleotides enzymatically
• Prepare DNA sequencing samples with one pipetting step
• Conserve precious PCR products with up to 100% recovery
• Minimize column cleanup and sample loss

The ExoSAP-IT one-step solution provides a simple way to enzymatically purify a PCR reaction. The reaction setup is complete with one pipetting step, which is followed by two incubations. The first incubation digests excess primer and dephosphorylates nucleotides. The second, high-temperature incubation inactivates the enzymes.

Enzymatic cleanup is a very efficient method for PCR purification. Adding ExoSAP-IT reagent directly to the PCR product eliminates transfer steps to tubes, wells, or columns and conserves PCR amplicons, helping reduce the chance of cross contamination; no further processing needed.

After enzymatic cleanup with ExoSAP-IT reagent, primers and dNTPs will no longer interfere with DNA sequencing or SNP analysis.

Specifications

For Use With (Application): PCR Purification
Concentration: 500 rxns
Description: ExoSAP-IT™ PCR Product Clean-up Reagent, 500 rxns, Recombinant, Dry Ice, DNA Sequencing or Single Nucleotide Polymorphism (SNP) Analysis, Use with PCR Purification, Store at –5 to °30°C
Quantity: 1mL
Protein Form: Recombinant
Research Category: Genetic Diseases
Shipping Condition: Dry Ice
Validated Application: PCR Purification
Product Line: ExoSAP-IT™

Contents & storage

Store at -5 to -30°C.