Applied Biosystems™
ExoSAP-IT™ PCR Product Cleanup Reagent
Description
ExoSAP-IT™ PCR Product Cleanup Reagent is used for enzymatic cleanup of amplified PCR product. It hydrolyzes excess primers and nucleotides in a single step. ExoSAP-IT-purified samples are ready for use in downstream applications such as DNA sequencing or single nucleotide polymorphism (SNP) analysis.• Remove excess primers and unincorporated nucleotides enzymatically
• Prepare DNA sequencing samples with one pipetting step
• Conserve precious PCR products with up to 100% recovery
• Minimize column cleanup and sample loss
The ExoSAP-IT one-step solution provides a simple way to enzymatically purify a PCR reaction. The reaction setup is complete with one pipetting step, which is followed by two incubations. The first incubation digests excess primer and dephosphorylates nucleotides. The second, high-temperature incubation inactivates the enzymes.
Enzymatic cleanup is a very efficient method for PCR purification. Adding ExoSAP-IT reagent directly to the PCR product eliminates transfer steps to tubes, wells, or columns and conserves PCR amplicons, helping reduce the chance of cross contamination; no further processing needed.
After enzymatic cleanup with ExoSAP-IT reagent, primers and dNTPs will no longer interfere with DNA sequencing or SNP analysis.
Specifications
| For Use With (Application): | PCR Purification |
|---|---|
| Concentration: | 5000 rxns |
| Description: | ExoSAP-IT™ PCR Product Clean-up Reagent, 5000 rxns, Recombinant, Dry Ice, DNA Sequencing or Single Nucleotide Polymorphism (SNP) Analysis, Use with PCR Purification, Store at –5 to °30°C |
| Quantity: | 10mL |
| Protein Form: | Recombinant |
| Research Category: | Genetic Diseases |
| Shipping Condition: | Dry Ice |
| Validated Application: | PCR Purification |
| Product Line: | ExoSAP-IT™ |
Contents & storage
Store at -5 to -30°C.
