New Protein Purification and Isolation Support Center—Find technical tips and troubleshooting recommendations from our scientists.
Thermo Scientific™

Pierce™ Glutathione Magnetic Agarose Beads

Catalog number:  78601

 Related applications:

Protein Purification & Isolation

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Thermo Scientific™ Pierce™ Glutathione Magnetic Agarose Beads provide a fast, convenient method for purification of glutathione-S-transferase (GST) from a bacterial, yeast, or mammalian crude cell lysate. Pierce™ Glutathione Magnetic Agarose Beads are well suited for purifying GST fusion proteins from a soluble protein extract and are ideal for the purification of proteins expressed at low levels from diluted supernatants. The beads can be used in manual applications with a magnetic stand or automated applications with an instrument such as the Thermo Scientific™ KingFisher™ Flex Magnetic Particle Processor. Automated instruments are especially useful for higher throughput purification and screening of purification conditions.

Download the KingFisher™ Duo BindIt™ Software protocol (.bdz) >
Download the KingFisher™ Flex BindIt™ Software protocol (.bdz) >

Features of Glutathione Magnetic Agarose Beads:
• High-performance beads—non-aggregating, magnetite (Fe3O4), superparamagnetic beads provide exceptional uniformity for both manual and automated HTS applications
• Stable affinity ligand—glutathione is covalently immobilized to particles, enabling leach-resistance and clean purification products
• High capacity—binding capacity is sufficient for both routine and demanding purification procedures

The high-performance, magnetite, superparamagnetic particles are validated and optimized for use with high-throughput magnetic platforms, such as the KingFisher™ 96 and KingFisher™ Flex magnetic particle processors, but the beads also enable premium performance for simple benchtop purification applications using an appropriate magnetic stand.

Glutathione S-transferase (GST) is a protein that is commonly added to the end of recombinant proteins to aid in their purification or detection. To make an affinity support, glutathione is immobilized through its central carbon, preserving the essential structure necessary for efficient GST binding. Addition of reduced glutathione in a physiologic buffer is sufficient to competitively displace and recover the GST fusion protein from the immobilized glutathione.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bulk or Custom Form: Bulk Custom Form 1
Format: Bottle(s)
Immobilized Phase: Glutathione
Product Line: Pierce™
Product Size: 1 mL
Shipping Condition: Wet Ice

Contents & storage

1 mL settled resin. Supplied as 4 mL of 25% v/v suspension in 20% ethanol.

Store at 2–8°C.

Documents

Manuals & protocols