Pierce™ Ni-NTA Magnetic Agarose Beads, 4 mL - FAQs

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4 product FAQs found

Are there other sequences that can bind to nickel more tightly than 6xHis-tagged proteins and how can they be eluted.

Yes, 7xHis-tagged proteins, proteins naturally high in histidine, and other combinations of His and other amino acids will bind. To elute them, you have to increase the concentration of imidazole. Generally these peptides will not contaminate your fraction since they remain on the column. However after multiple uses of the same column, these peptides may reduce the binding capacity of the column.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can the Pierce Magnetic Agarose Beads be frozen?

No. Freezing will adversely affect the agarose structure and may cause bead aggregation or loss of binding.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can the Pierce Magnetic Agarose Beads be used in an automated magnetic particle processor?

Yes. Protocols for using the beads on a KingFisher instrument are available on the product page.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What advantage do Pierce Magnetic Agarose beads have over Dynabeads or Pierce Magnetic Beads?

Pierce Magentic Agarose Beads have a much higher binding capacity.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.