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TNF (TNFA) Recombinant Rat Protein

Catalog number: 80045RNAE50

 Related applications: Protein Assays and Analysis

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Description

Tumor Necrosis Factor (TNF), recombinant rat protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications

This recombinant protein was expressed from a DNA sequence encoding the mature form of rat TNF-alpha (P 16599) (Val 80-Leu 235) fused to an initial Met at the N-terminus.

Activity: Measured in a cytotoxicity assay using L930 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 is typically 10-20 pg/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Apoptosis, Cell Cycle & Proliferation, Inflammation, Oncology
Expression: E. coli
Form: Lyophilized
Gene Aliases: Tnf, MGC124630, RATTNF, TNF-alpha, Tnfa
Gene Symbol: TNF
Molecular Weight: 17.4 kDa
Product Line: Sino Biological®
Product Size: 50 µg
Protein Family: Cytokines & Receptors
Protein Name: tumor necrosis factor
Protein Subtype: TNF (Tumor Necrosis Factor)
Protein Tag or Fusion: Untagged
Purity: ≥ 90% by SDS-PAGE
Species: Rat
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

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Manuals & protocols

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