The CaptureSelect IgE Ligand Leakage ELISA detects possible IgE affinity ligand contamination of the purified product after use of CaptureSelect IgE Affinity Matrix.
The CaptureSelect IgE affinity ligand is a 13-kD recombinant protein, expressed in yeast and covalently coupled to a chromatography matrix for high chemical stability and low leakage. During the chromatography process, small amounts of affinity ligand can leach off of the chromatography support and co-elute with the protein. This sensitive ELISA detects affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation even in the presence of the protein of interest. The CaptureSelect IgE Ligand Leakage ELISA can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.
For Research Use Only. Not for use in diagnostic procedures.