Thermo Scientific Pierce 1-Step Transfer Buffer is designed for rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.
Features of 1-Step Transfer Buffer:
• Fast—high ionic strength formulation provides 5- to 10-minute protein transfer when used with compatible fast semi-dry blotting systems • Ready to use—supplied as a 1X solution that is stable and ready to use at room temperature • Optimized—designed for seamless and reliable performance with the Pierce G2 Fast Blotter • Compatible—effective with other semi-dry transfer devices equipped with a suitable high-current power supply, including the original Pierce Fast Semi-Dry Blotter (Part No. 88217) • Economical—excellent performance without the special or costly consumables that are required by other fast semi-dry transfer devices
When used with the Pierce G2 Fast Blotter, this buffer provides effective gel-to-membrane transfer of proteins in 5 to 10 minutes with efficiency that is equal to, or better than, conventional Western blotting techniques. Pierce 1-Step Transfer Buffer is also compatible with other protein semi-dry transfer devices, including the original Pierce Fast Semi-Dry Blotter (Part No. 88217), when they are paired with a suitable high-current power supply. Such devices provide constant high current (1.3 to 5.0 amps) to rapidly transfer proteins via the high ionic strength conditions supplied by the transfer buffer.
Fast blotting methods require a high-current power supply, such as the Pierce G2 Fast Blotter, and an optimized high ionic strength transfer buffer, such as Pierce 1-Step Transfer Buffer. By increasing the current, excellent transfer efficiency can be achieved in much shorter time compared to conventional methods. Amperage is held at a constant rate based on the surface area of the transfer stack(s) (~22-23mA/cm2) and voltage is limited to 25V.
For Research Use Only. Not for use in diagnostic procedures.