Thermo Scientific™

SuperSignal™ Molecular Weight Protein Ladder

Catalog number: 84785
Thermo Scientific™

SuperSignal™ Molecular Weight Protein Ladder

Catalog number: 84785
Catalog Number
Unit Size
250 µL
Price (USD)
Catalog NumberUnit SizePrice (USD)Quantity
84785250 µL
Product Overview
Citations & References
Additional Information
Thermo Scientific SuperSignal Molecular Weight Protein Ladder (20 to 150K) is a mix of eight unstained recombinant proteins with IgG-binding sites for chemiluminescent, fluorescent, chromogenic or other detection systems. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Compare and view all other protein standards and ladders ›

The ladder’s recombinant proteins bind to the antibodies used in the western blot through the IgG binding site. The protein markers can then be visualized using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel).

Two versions of the MW marker are offered. This formulation is appropriate for use with most rabbit and other non-mouse polyclonal antibodies. SuperSignal Enhanced Molecular Weight Protein ladder is formulated specifically for applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used. For example, the enhanced standard may be necessary when using a 1:50,000 (25 ng/mL) to 1:100,000 (10 ng/mL) dilution of secondary antibody.

For Research Use Only. Not for use in diagnostic procedures.


Detection Method
User defined detection system
Number of Markers
Product Type
Protein Ladder
Ready to Load
Size Range
20 to 150 kDa
Stain Type
Gel Compatibility
Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
Molecular Weight (g/mol)
150, 100, 80, 60, 50, 40, 30, 20 kDa
Product Line
250 μL
Shipping Condition
Wet Ice
System Type
Western Blotting, SDS-PAGE

Contents & Storage

Contents: one vial of 250 μL

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Storage: Upon receipt store at -20°C for long term storage, or three months at 4°C or one month at ambient


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