Thermo Scientific Pierce LDS Sample Loading Buffer (4X) is a nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes.
Features of LDS Sample Loading Buffer:
• Concentrated—4X formulation provides more versatility than traditional 2X buffers • Nonreducing—ready to use for non-reducing SDS-PAGE, or the preferred type and amount of reducing agent (e.g., DTT) can be added to produce reducing conditions • SDS alternative—lithium dodecyl sulfate (LDS) replaces sodium dodecyl sulfate (SDS) of traditional loading buffers but functions equally well • Physiologic pH—buffered with triethanolamine (pH 7.6) rather than Tris (pH 6.8) • Lane marker dyes—includes trace amounts of phenol red and coomassie G250 as visible markers of the dye front • Convenient—stable at room temperature, enabling storage at the bench where electrophoresis is performed
LDS Sample Buffer, Non-Reducing (4X) is a convenient sample buffer for use in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The buffer contains coomassie dye, enabling visualization of the electrophoretic progress by the location of the dye front. The LDS Sample Buffer, Non-Reducing (4X) may be used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.