Pierce™ Mass Spec Sample Prep Kit for Cultured Cells

Catalog number: 84840

Thermo Scientific™  Related applications: Protein Mass Spectrometry Analysis

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Description

The Thermo Scientific Pierce Mass Spec Sample Prep Kit for Cultured Cells is an easy-to-use, comprehensive kit for preparation of clean peptide mixtures from cultured cells for mass spectrometry (MS) analysis.

Features of the Mass Spec Sample Prep Kit:

Complete—includes all reagents, a digestion indicator control, proteases and an optimized protocol needed to process up to 20 samples
Simple—user friendly kit can provide reproducible results even for non-expert MS analysts
Flexible—can be adapted to handle sample sizes between 10 to 200 µg
High yield—total protein yield from 1 million cells is greater than 100 µg
Optimized—cysteine reduction and alkylation are 100% with less than 1% over alkylation of non-cysteine residues
Efficient - percentage of missed cleavages is less than 10%
Compatible—final preparation is ready for direct MS analysis and other downstream applications, including mass-tag labeling

The Pierce Mass Spec Sample Prep Kit for Cultured Cells contains all the necessary reagents and enzymes to prepare up to 20 samples (1 million cells each) for MS analysis. The simple and robust workflow uses a lysis protocol that generates approximately 100 µg of protein per sample and includes a control digestion indicator to monitor the efficiency of the two-step enzymatic digestion protocol. The kit procedure, which includes a Digestion Indicator as an internal protein/peptide control, produces consistently and reproducibly clean peptide mixtures for protein identification.

Includes:
Kit contains proprietary lysis and digestion buffers, digestion indicator, DTT, iodoacetamide, Lys-C, trypsin

Sample prep for mass spectrometry (MS, mass spec) remains one of the largest bottlenecks associated with MS analysis, and consistent and reproducible sample preparation can make the difference between a successful analysis and a failed outcome. Current sample prep protocols are primarily homebrew and can be highly variable, making data analysis and interpretation difficult. The Mass Spec Sample Prep Kit for Cultured Cells provides researchers with all the necessary tools to generate consistent and reproducible protein digests that are directly compatible with LC-MS workflows. The digests do not require further processing, such as C18 clean up or detergent removal.

The included Pierce Digestion Indicator, which can also be purchased separately, is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples. The protein sequence and recommended peptides to monitor across samples are supplied with the product instructions.

 

 

 

More Product Data
A versatile mass spectrometry sample preparation procedure for complex protein samples

Related Products
Pierce™ Digestion Indicator for Mass Spectrometry
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 20 reactions
Final Product: Peptide
Workflow Step: Protein Digestion, Cell Lysis, Sample Clean Up
Detection Method: Mass Spectrometry
Starting Material: Mammalian cells
For Use With (Equipment): Mass Spectrometer, Microcentrifuge

Contents & storage

Upon receipt, remove Insert A (containing Pierce Digestion Indicator, Lys-C Protease and Pierce Trypsin Protease, MS Grade) and store at -20°C. Store the remaining components at 4°C. Product is shipped on dry ice.

Documents

Manuals & protocols