Acetonitrile (ACN), LC-MS Grade - FAQs

What kind of buffers and solutions are compatible with peptide clean-up and LC-MS?

Most organic solvents or volatile buffers are compatible with LC-MS. Water and acetonitrile with 0.1% formic acid is the most common solvent system for separation of peptide samples for mass spectrometry analysis. Trifluoracetic acid (TFA) is an alternative ion pairing agent for off-line peptide C18 clean-up or LC-UV analysis. We offer the following LC-MS grade reagents: Pierce 0.1% Trifluoroacetic Acid (v/v) in Water (Cat. No. 85172), Pierce Water, LC-MS Grade (Cat. No. 85189), Pierce Acetonitrile (ACN) (Cat. No. 85188), and Pierce Trifluoroacetic (TFA) (Cat. No. 85183).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.