Human PrimeFlow™ RNA Assay

Catalog number: 88-18009-204

Invitrogen™  Related applications: Cellular Imaging | Flow Cytometry | miRNA & Non-Coding RNA Analysis

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Description

This Human PrimeFlow™ RNA Assay (3-plex) is an in situ hybridization assay that combines the power of branched-DNA technology with the single cell resolution of flow cytometry to detect up to three RNA targets in a single sample. Branched-DNA technology uses pairs of target-specific probes to achieve up to 8,000-16,000 fold signal amplification from a desired RNA transcript. This assay allows simultaneous detection of RNA targets in combination with cell surface and intracellular staining using fluorochrome-conjugated antibodies to allow further discrimination of specific cell subpopulations.

This Human PrimeFlow RNA Assay kit contains all the reagents needed to conduct the assay, including a set of positive control probes for human ribosomal protein, RPL13A (NM_012423). Target Probe sets for genes of interest are sold separately.

For the study of mouse samples, use the Mouse PrimeFlow RNA Assay (3-plex, cat. 88-18001). For the study of alternative species, either kit may be used, however, appropriate positive control probes need to be purchased separately.




Reactivity/Species
Human

Reported Application
Flow Cytometric Analysis

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 40 tests

Documents

Manuals & protocols