Human PrimeFlow™ RNA Assay

Catalog number:  88-18009-204

Invitrogen™  Related applications: Cellular Imaging | Flow Cytometry | miRNA & Non-Coding RNA Analysis

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This Human PrimeFlow™ RNA Assay (3-plex) is an in situ hybridization assay that combines the power of branched-DNA technology with the single cell resolution of flow cytometry to detect up to three RNA targets in a single sample. Branched-DNA technology uses pairs of target-specific probes to achieve up to 8,000-16,000 fold signal amplification from a desired RNA transcript. This assay allows simultaneous detection of RNA targets in combination with cell surface and intracellular staining using fluorochrome-conjugated antibodies to allow further discrimination of specific cell subpopulations.

This Human PrimeFlow RNA Assay kit contains all the reagents needed to conduct the assay, including a set of positive control probes for human ribosomal protein, RPL13A (NM_012423). Target Probe sets for genes of interest are sold separately.

For the study of mouse samples, use the Mouse PrimeFlow RNA Assay (3-plex, cat. 88-18001). For the study of alternative species, either kit may be used, however, appropriate positive control probes need to be purchased separately.


Reported Application
Flow Cytometric Analysis

For Research Use Only. Not for use in diagnostic procedures.


Product Size: 40 tests


Manuals & protocols