ViewRNA™ Cell Plus Assay Kit

Citações e referências (10)

Invitrogen™

ViewRNA™ Cell Plus Assay Kit

The ViewRNA Cell Plus Assay is a novel assay that combines immunocytochemistry with ViewRNA technology, a proprietary fluorescent in situLeia mais

Número do catálogo	Quantity
88-19000-99	1 kit

Número do catálogo 88-19000-99

Preço (BRL)

19.166,94

Adicionar ao carrinho

Quantity:

1 kit

Preço (BRL)

19.166,94

Adicionar ao carrinho

The ViewRNA Cell Plus Assay is a novel assay that combines immunocytochemistry with ViewRNA technology, a proprietary fluorescent in situ hybridization and sequential branched-DNA amplification technique, to visualize both RNA with single-molecule sensitivity and protein in individual cells. This assay enables simultaneous detection of up to three RNA targets in combination with immunophenotyping for cell surface or intracellular proteins using both indirect and direct immunocytochemistry to allow for detailed characterization of specific cell subpopulations. For simultaneous detection of a fourth RNA target, the ViewRNA ISH Cell 740 Module (Cat. No. QVC0200) can be combined with this kit. It allows analysis of an addtional target in the 740 channel (AlexaFluor 750).

This ViewRNA Cell Plus Assay kit contains all the reagents needed to conduct the assay. Target Probe sets for genes of interest and antibodies are sold separately.

Reactivity/species
Probes can be designed for any species. These are the species most frequently used/tested: Armenian hamster, baboon, bovine, canine, cat, chicken, chimpanzee, cow, cynomolgus monkey, donkey, goat, golden Syrian hamster, guinea pig, hamster, horse, human, macaque, monkey, mouse, non-human primate, olive baboon, pig, pigtailed macaque, rabbit, rat, rhesus monkey, sheep

Reported application
Microscopy, immunocytochemistry

For Research Use Only. Not for use in diagnostic procedures.

Especificações

For Use With (Application)Microscopy, Immunocytochemistry

Product TypeRNA In Situ Hybridization Assay Kit

Quantity1 kit

Detection MethodPrimer-probe Detection

FormatKit

Unit SizeEach

Frequently asked questions (FAQs)

How do ViewRNA assays compare to RNAScope assays?

ViewRNA and RNAScope technologies rely on the same signal amplification strategy - branched DNA amplification. Historically, both ViewRNA and RNAScope technologies originated from the same company, Panomics. The assays are expected to yield similar sensitivity and resolution, however each technology relies on its own set of proprietary reagents and probe set designs. Hence, the assays are not considered interchangeable or compatible. ViewRNA probe sets are not tested for RNAScope assaya and vice versa.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is the signal amplified in ViewRNA assays?

The ViewRNA technology relies on branched DNA signal amplification strategy. Target probes complementary to the target transcript sequence are further hybridized with pre-amplifier, amplifier and label probes that consist of branched DNA, and form 'tree branches' that allow numerous label probes to attach. This approach allows higher signal amplification compared to traditional ISH techniques.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Where can I find general information about ViewRNA ISH Assays?

For general information about ViewRNA ISH Assays, please go to this page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/in-situ-hybridization-ish/rna-fish/viewrna-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For ViewRNA assays, can I use the same set of wash solutions for all samples, including the positive and negative controls?

We do not recommend doing this. The negative control should be processed and washed separately from the rest of the samples. This is because the negative control does not contain any target probe sets and only the amplification reagents are added to it. If experimental samples are washed in the same beaker of wash solutions as the negative control, any unbound target probes that wash away can carry over to the negative control sample and cause unexpected positive signal (that will also appear to be very specific).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

To perform ViewRNA assays, which incubator oven should I use, Cat. No. QS0704 or QS0712?

Cat. No. QS0704 is a 120 V unit, for use in US/Canada region (https://www.thermofisher.com/order/catalog/product/QS0704).

Cat. No. QS0712 is a 220 V unit, for use in European region (https://www.thermofisher.com/order/catalog/product/QS0712).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all ViewRNA™ Cell Plus Assay Kit FAQs

Citações e referências (10)

Citações e referências

Abstract

Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes.

Authors:Rheaume BA, Jereen A, Bolisetty M, Sajid MS, Yang Y, Renna K, Sun L, Robson P, Trakhtenberg EF

Journal:Nat Commun

PubMed ID:30018341

'Retinal ganglion cells (RGCs) convey the major output of information collected from the eye to the brain. Thirty subtypes of RGCs have been identified to date. Here, we analyze 6225 RGCs (average of 5000 genes per cell) from right and left eyes by single-cell RNA-seq and classify them into 40 ... More

miR-103 promotes endothelial maladaptation by targeting lncWDR59.

Authors:Natarelli L, Geißler C, Csaba G, Wei Y, Zhu M, di Francesco A, Hartmann P, Zimmer R, Schober A

Journal:Nat Commun

PubMed ID:29980665

'Blood flow at arterial bifurcations and curvatures is naturally disturbed. Endothelial cells (ECs) fail to adapt to disturbed flow, which transcriptionally direct ECs toward a maladapted phenotype, characterized by chronic regeneration of injured ECs. MicroRNAs (miRNAs) can regulate EC maladaptation through targeting of protein-coding RNAs. However, long noncoding RNAs (lncRNAs), ... More

Long non-coding RNAs influence the transcriptome in pulmonary arterial hypertension: the role of PAXIP1-AS1.

Authors:Jandl K, Thekkekara Puthenparampil H, Marsh LM, Hoffmann J, Wilhelm J, Veith C, Sinn K, Klepetko W, Olschewski H, Olschewski A, Brock M, Kwapiszewska G

Journal:J Pathol

PubMed ID:30450722

'In idiopathic pulmonary arterial hypertension (IPAH), global transcriptional changes induce a smooth muscle cell phenotype characterised by excessive proliferation, migration, and apoptosis resistance. Long non-coding RNAs (lncRNAs) are key regulators of cellular function. Using a compartment-specific transcriptional profiling approach, we sought to investigate the link between transcriptional reprogramming by lncRNAs ... More

Detection and Differentiation of Multiple Viral RNAs Using Branched DNA FISH Coupled to Confocal Microscopy and Flow Cytometry.

Authors:van Buuren N, Kirkegaard K

Journal:Bio Protoc

PubMed ID:30505886

Due to the exceptionally high mutation rates of RNA-dependent RNA polymerases, infectious RNA viruses generate extensive sequence diversity, leading to some of the lowest barriers to the development of antiviral drug resistance in the microbial world. We have previously discovered that higher barriers to the development of drug resistance can ... More

KDM6A and KDM6B play contrasting roles in nuclear transfer embryos revealed by MERVL reporter system.

Authors:Yang L, Song L, Liu X, Bai L, Li G

Journal:EMBO Rep

PubMed ID:30389724

Despite the success of animal cloning by somatic cell nuclear transfer (SCNT) in many species, the method is limited by its low efficiency. After zygotic genome activation (ZGA) during mouse development, a large number of endogenous retroviruses (ERVs) are expressed, including the murine endogenous retrovirus-L (MuERVL/MERVL). In this study, we ... More

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