Invitrogen™

CaspGLOW™ Fluorescein Active Caspase Staining Kit

Catalog number:  88-7003-42

 Related applications: Flow Cytometry

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Description

The CaspGLOW™ Fluorescein Active Staining Kit contains the reagents to sensitively detect active caspases in mammalian cells. This assay utilizes the inhibitor specific for all caspases, Z-VAD-FMK, that is directly conjugated to FITC (fluorescein isothiocyanate) for the detection system. This reagent is cell permeable, non-toxic and irreversibly binds to the active enzyme. Detection of the labeled cells can be determined by flow cytometry, fluorescent microscopy or a fluorescent plate reader.

The Caspases are a family of aspartate-specific cysteine proteases that act in a step-wise signaling manner like kinases. Recruitment of these proteases to oligomerized receptors leads to activation and autoproteolytic cleavage. Active caspases can proteolyze additional caspases generating a caspase cascade. The final outcome of this signaling pathway is a form of controlled cell death, termed apoptosis.

Reported Application
Flow Cytometric Analysis, Immunocytochemistry

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Fluorescent
For Use With (Equipment): Flow Cytometer, Fluorescence Microscope
Format: Kit
Product Size: 100 tests

Documents

Manuals & protocols