Invitrogen™

CCL4 Human Uncoated ELISA Kit with Plates

Catalog number:  88-7034-86

 Related applications:

Protein Assays and Analysis

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Description

This Human CCL4 (MIP-1 beta) Uncoated ELISA Kit with Plates contains the necessary reagents, standards, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA). This ELISA kit is specifically engineered for accurate and precise measurement of protein levels from samples including serum, plasma, and supernatants from cell cultures.

CCL4, also known as MIP-1 beta (Macrophage Inflammatory Protein-1 beta), is a member of the CC-subfamily of chemokines and is most closely related to CCL3, or MIP-1 alpha. These proteins play critical roles in the recruitment of leukocytes to the site of inflammation. While both CCL3 and CCL4 are chemoattractant for monocytes, macrophages, and dendritic cells, CCL4 preferentially attracts CD4+ T cells, while CD8+ T cells are more responsive to CCL3. In addition to its chemotactic functions, CCL4 induces the release of proinflammatory cytokine, mast cells degranulation, and NK cell activation.

CCL4 signaling is mediated by the G protein-coupled receptors CCR1, CCR4, and CCR5, which are shared with CCL3 and CCL5 (RANTES). CCR5 is the primary co-receptor for HIV entry, which the virus binds through the gp120 envelope protein. All CCR5 ligands demonstrate potent inhibition of virus entry into the cell, both through steric hindrance of gp120-CCR5 interaction, and ligand-mediated receptor internalization.

Reported Application
ELISA
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Range: 4-500 pg/mL
Assay Sensitivity: 4 pg/mL
Detection Method: Colorimetric
Detector Antibody Conjugate: Biotin
For Use With (Equipment): Microplate Reader
Former Identifiers: Ready-SET-Go!, eBioscience
Gene Aliases: ACT2, AT744.1, G-26, HC21, LAG-1, LAG1, MIP-1-beta, MIP1B, MIP1B1, SCYA2, SCYA4
Gene ID: 6351
Gene Name: C-C motif chemokine ligand 4
Gene Symbol: CCL4
Immunoassay Kit Format: Uncoated ELISA Kit with Plates
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 10 x 96 tests
Protein Name: C-C motif chemokine 4
Target (Kit Named): CCL4
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P13236

Contents & storage

Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer: Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme
Enzyme: Pre-titrated Streptavidin-HRP
Substrate: 1X TMB Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22, -76, -86)

Documents

Manuals & protocols