Notice: This product will be discontinued once remaining inventory is depleted.
Invitrogen™

IL-21 Mouse ELISPOT Kit

Catalog number:  88-7210-88

Related applications:

Protein Assays and Analysis

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Description

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by NKT, T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl-6. As with IFN gamma for Th1, IL-4 for Th2 cells, and IL-17A for Th17 cells, IL-21 is critical for TFH effector function. This cytokine plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naïve B cells.

In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. High levels of IL-21 are present in chemically-induced colitis models. IL-21-deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules.

Reported Application
ELISPOT

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Range: See product documentation
Gene ID (Entrez): 60505
Assay Sensitivity: See product documentation
Detector Antibody Conjugate: Biotin
Gene Symbol: IL21
Former Identifiers: Ready-SET-Go!, eBioscience
Gene Alias: IL-21
Gene: interleukin 21
Protein Aliases: IL-21
Protein: Interleukin-21
Target Kit Named: IL-21
Target Species Primary: Mouse
Target Species Named: Mouse
UniProt ID: Q9ES17
Label or Dye: HRP (Horseradish Peroxidase)
Immunoassay Kit Format: ELISPOT Kit
Target Species Validated: Mouse
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader

Contents & storage

Capture Antibody: Pre-titrated, Functional Grade (low endotoxin) purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
ELISA/ELISPOT Coating Buffer: This ELISPOT Kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM)) or 10X PBS ELISPOT Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20 and filter with 0.22 µM).
Assay Diluent: 5X Concentrated
Detection Enzyme: Pre-titrated Avidin-HRP
Certificate of Analysis: Lot-specific instructions for the dilution of antibodies and enzyme