TNF alpha Mouse Uncoated ELISA Kit with Plates

Catalog number:  88-7324-22

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

This Mouse TNF alpha Uncoated ELISA Kit with Plates contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA kit is specifically engineered for accurate and precise measurement of mouse TNF alpha protein levels from samples including serum, plasma, tissue homogenate, lavage fluid and supernatants from cell cultures. Recognition of mouse TNF alpha by this assay is not interfered with by 1000X excess soluble TNF alpha receptor type I or II.

Reported Application
ELISA

For Research Use Only. Not for use in diagnostic procedures.

Specifications

LoyaltyEligible: SKUEligibleForLoyalty
Assay Range: 8-1,000 pg/mL
Assay Sensitivity: 8 pg/mL
Detection Method: Colorimetric
Detector Antibody Conjugate: Biotin
For Use With (Equipment): Microplate Reader
Former Identifiers: Ready-SET-Go!, eBioscience
Gene Aliases: DIF, TNF-a, TNF-alpha, TNFSF2, TNFalphaa, Tnfsf1a, Tnlg1f, Tnf
Gene ID: 21926
Gene Name: tumor necrosis factor
Gene Symbol: TNF
Immunoassay Kit Format: Uncoated ELISA Kit with Plates
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 2 x 96 tests
Protein Name: tumor necrosis factor
Target (Kit Named): TNF alpha
Target Species (Named): Mouse
Target Species (Validated): Mouse
UniProt ID: P06804

Contents & storage

Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit with plates may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent: 5X concentrated
Detection enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22, -44, -76, -86)

Documents

Manuals & protocols