eBioscience™ Annexin V Apoptosis Detection Kits
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Invitrogen™

eBioscience™ Annexin V Apoptosis Detection Kits

Kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane.
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Catalog NumberQuantityExcitation/EmissionConjugate
88-8103-7250 TestsPE/Cy7
88-8005-74200 Tests488, 535/520, 617FITC, Propidium Iodide
88-8006-7250 Tests405, 546/450, 647eFluor 450, 7-AAD
88-8006-74200 Tests405, 546/450, 647eFluor 450, 7-AAD
88-8007-74200 Tests650, 535/660, 617APC, Propidium Iodide
88-8008-7250 TestsPCP-eFluor 710
88-8008-74200 TestPCP-eFluor 710
BMS500FI-100100 Tests488, 535/520, 617FITC, Propidium Iodide
BMS500FI-2020 Tests488, 535/520, 617FITC, Propidium Iodide
BMS500FI-300300 Tests488, 535/520, 617FITC, Propidium Iodide
88-8102-7250 TestsPE, 7-AAD
88-8102-74200 TestsPE, 7-AAD
88-8103-74200 TestsPE/Cy7
BMS500BT-100
also known as BMS500BT/100
100 TestsNA, 535/NA, 617Biotin, Propidium Iodide
BMS500BT-20
also known as BMS500BT/20
20 TestsNA, 535/NA, 617Biotin, Propidium Iodide
BMS500BT-300
also known as BMS500BT/300
300 TestsNA, 535/NA, 617Biotin, Propidium Iodide
88-8005-7250 Tests488, 535/520, 617FITC, Propidium Iodide
88-8007-7250 Tests650, 535/660, 617APC, Propidium Iodide
Catalog number 88-8103-72
Price (USD)/ Each
289.00
Add to cart
Quantity:
50 Tests
Conjugate:
PE/Cy7
Price (USD)/ Each
289.00
Add to cart
Easily distinguish cells undergoing early and late apoptosis with eBioscience and Annexin V Apoptosis Detection kits for flow cytometry analysis. These kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane. In late-stage apoptosis, as cell integrity is lost, both annexin V and the viability dye traverse and bind PS on the interior of the plasma membrane.
Annexins are a family of calcium-dependent phospholipid-binding proteins that preferentially bind phosphatidylserine (PS). Under normal physiologic conditions, PS is predominantly located in the inner leaflet of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution across the phospholipid bilayer and is translocated to the extracellular membrane leaflet marking cells as targets of phagocytosis. Once on the outer surface of the membrane, PS can be detected by fluorescently labeled annexin V in a calcium-dependent manner.

In early-stage apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD, or fixable viability dyes such as eFluor 450. These cells will stain with annexin V but not a viability dye, thus distinguishing cells in early apoptosis. However, in late-stage apoptosis, the cell membrane loses integrity, thereby allowing annexin V to also access PS in the interior of the cell. A viability dye can be used to resolve these late-stage apoptotic and necrotic cells (annexin V, viability dye-positive) from the early-stage apoptotic cells (annexin V positive, viability dye-negative).

Current viability stains available with the eBioscience Annexin V Apoptosis Detection kits include FITC, eFluor 450, allophycocyanin (APC), phycoerythrin (PE), PE-cyanine7, and biotin.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptioneBioscience Annexin V Apoptosis Detection Set PE-Cyanine7
For Use With (Equipment)Flow Cytometer
Kit ContentsContains:
• Annexin V Binding Buffer (10X), 1 bottle (30 mL)
• Annexin V PE-Cyanine7, 1 vial (0.25 mL)

Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
Product TypeApoptosis Detection Kit
Quantity50 Tests
Shipping ConditionWet Ice
ConjugatePE/Cy7
Unit SizeEach
Contents & Storage
Handling Conditions:
Store at 2-8°C. Avoid temperature fluctuations.