This human Th17 cytokine staining panel includes all reagents needed for simultaneous flow cytometric detection of all the major cytokines produced by the Th17 lineage: IL-17A, IL-17F, IL-21 and IL-22. An anti-CD4 antibody that can be used after fixation and permeabilization, as well as IC Fixation and Permeabilization Buffers, is also included.
CD4+ T helper cells are critical mediators of the cellular immune response. For many years, due to cytokine expression patterns, it was thought that CD4+ T helper cells existed as a dichotomy of lineages named Th1 and Th2. However, further investigation revealed that the T helper cell population was not limited to these two subsets. Although it had long been appreciated that IL-17 (also known as IL-17A) production by T cells was required for protection against some pathogens, studies demonstrated that this cytokine was produced by a unique subset of T helper cells. Subsequent reports definitively showed that T cells could differentiate into IL-17-producing cells in vitro and in vivo independent of Th1 or Th2 development, thereby establishing Th17 cells as a unique T helper cell lineage. In addition to IL-17A expression, Th17 cells have been reported to express IL-17F (an heterodimers of IL-17A and Il-17F), IL-21 and IL-22. Functionally, Th17 cells play a role in host defense against extracellular pathogens by mediating the recruitment of neutrophils and macrophages to infected tissues. Moreover, it is becoming evident that aberrant regulation of Th17 cells may play a significant role in the pathogenesis of multiple inflammatory and autoimmune disorders.
Intracellular Staining Followed by Flow Cytometric Analysis
For Research Use Only. Not for use in diagnostic procedures.