Invitrogen™

eBioscience™ BrdU Kit for IHC/ICC Colorimetric

Catalog number:  8800-6599-45

 Related applications:

Cellular Imaging

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Description

This BrdU Kit for immunohistochemistry (IHC)/immunocytochemistry (ICC) with colorimetric detection contains the necessary reagents and buffers for identifying and examining proliferating cells by immunohistochemical or immunocytochemical analysis. Cells are labeled in vitro or in vivo with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine, which is incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Following fixation and antigen retrieval steps, cells or tissue sections are stained for BrdU incorporation and visualized using an Avidin-HRP (horseradish peroxidase) and DAB (3, 3'-Diaminobenzidine) substrate reaction. This kit has been optimized for IHC with both frozen and paraffin embedded BrdU-labeled mouse intestine and ICC of BrdU-pulsed HeLa cells grown on culture slides.

Reported Application
Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections, Microscopy, Immunocytochemistry, Immunohistochemical Staining of Frozen Tissue Sections

WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Colorimetric
Product Line: eBioscience™
Product Size: 50 tests
Reactivity: Mouse, Chimpanzee, Human, Macaque, Rat
Substrate: DAB
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Target Species: All species
Target Specificity: BrdU
Technique: ICC (Immunochemistry), IHC (Immunohistochemistry)

Documents

Manuals & protocols