Thermo Scientific™

Nitrocellulose Membrane, 0.45 μm, 30 cm x 3.5 m

Catalog number:  88018

Related applications:

Protein Electrophoresis & Western Blotting

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Pierce Nitrocellulose Membrane, 0.45 µm, comes in a convenient 30 cm x 3.5m roll and has excellent binding properties for western blotting, dot-blot assays, and other protein or nucleic acid methods. The 0.45-µm pore size is ideal for the transfer of most proteins (>20 kDa) and nucleic acids (>300 bp).

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Membrane: 100% pure nitrocellulose
Binding capacity: ~80 µg/cm2 of proteins
Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• Economical—30 cm x 3.5 m roll for cutting to any dimensions
For Research Use Only. Not for use in diagnostic procedures.


Membrane: Nitrocellulose Membrane
Quantity: 1 Roll
Sufficient For: At least 120 mini-gel blots when cut to 8 x 10 cm
Material: 100% nitrocellulose paper
Pore Size: 0.45 micron
Description: Pierce Nitrocellulose Membrane, 0.45um, 30cm x 3.5m
Dimensions (L x W): 30 cm x 3.5 m

Contents & storage

Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure. Keep membranes out of direct sunlight. Sunlight may cause the nitrocellulose to dry and become brittle. Note: Nitrocellulose membrane filters are highly flammable. Keep away from heat and open flame. Flash point is approximately 2°C. Membranes can be sterilized by steam-autoclaving at 121°C.