Invitrogen™

MagniSort™ Human CD4 T cell 2-Step Enrichment Kit

Catalog number:  8802-6831-74

 Related applications:

Cell Isolation & Expansion

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Description

The MagniSort™ Human CD4 2-Step Enrichment Kit is designed for the magnetic separation of CD4+ T cells by positive selection after first depleting monocytes. It has been optimized for the isolation of CD4+ T cells from human peripheral blood monocuclear cells, utilizing biotinylated Anti-Human CD14 and Anti-Human CD4 antibodies, as well as streptavidin-coated magnetic beads. In the first step, monocytes are bound by Anti-Human CD14 antibody and then magnetic beads. When placed in a magnetic field, the monocytes are held in the magnet while the remaining cells are decanted and used for the second step. In the second step, the monocyte-depleted cells are bound by Anti-Human CD4 antibody and then magnetic beads. Now, when placed in the magnetic field, the CD4+ T cells are held in the magnet while undesired cells can be decanted.

After enrichment, the purity of selected cells can be verified by staining with Anti-Human CD4, clones OKT4 or SK3.

Reported Application
Magnetic Cell Separation

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: CD4+
Product Size: 200 tests
Target Species: Human

Documents

Manuals & protocols