Thermo Scientific™

Nitrocellulose Membranes, 0.45 µm, 8 x 8 cm

Catalog number:  88025

Related applications:

Protein Electrophoresis & Western Blotting

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Pierce Nitrocellulose Membranes, 0.45 µm, 8 x 8 cm, have excellent binding properties for western blotting, dot-blot assays, and other protein or nucleic acid methods. The 0.45-µm pore size is ideal for the transfer of most proteins (>20 kDa) and nucleic acids (>300 bp).

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Media: 100% pure nitrocellulose
Binding capacity: ~45-60 µg/cm2 of BSA
Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
For Research Use Only. Not for use in diagnostic procedures.


Membrane: Nitrocellulose Membrane
Pore Size: 0.45 micron
Dimensions (L x W): 8 cm x 8 cm
Sufficient For: 15 mini-gel blots
Quantity: 15 Sheets
Gel Size: one mini gel per sheet

Contents & storage

Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure. Keep membranes out of direct sunlight. Sunlight may cause the nitrocellulose to dry and become brittle.
Note: Nitrocellulose membrane filters are highly flammable. Keep away from heat and open flame. Flash point is approximately 2°C. Membranes can be sterilized by steam-autoclaving at 121°C.