Invitrogen™

MagniSort™ Mouse Hematopoietic Lineage Depletion Kit

Catalog number:  8804-6829-74

 Related applications:

Cell Isolation & Expansion

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Description

The MagniSort™ Mouse Hematopoietic Lineage Depletion Kit is designed for the magnetic depletion of mouse hematopoietic cells with committed lineages from mouse bone marrow. It utilizes a cocktail of antibody specific for hematopoietic lineages and streptavidin-coated magnetic beads. Lineage committed cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the lineage negative cells can be separated from lineage committed cells by decanting.

The MagniSort™ Mouse Hematopoietic Lineage Depletion Antibody Cocktail contains the following antibodies:
Anti-Mouse CD2 Biotin
Anti-Mouse CD3 Biotin
Anti-Mouse CD5 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD45R Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse TER-119 Biotin

After depletion, the enrichment of multi-potent progenitor cells can be verified by staining with Anti-Mouse CD34 (clone RAM34), and the enrichment of CD34- hematopoietic stem cells can be verified by additional staining with Anti-Mouse CD150 (clone mShad150) and Anti-Mouse CD48 (clone HM48-1).

Reported Application
Magnetic Cell Separation

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: Hematopoietic Cells
Product Size: 200 tests
Target Species: Mouse

Documents

Manuals & protocols