Note: This product will be discontinued soon. Its replacement, catalog #88370 (DMEM:F-12 for SILAC) is available for online purchase now.

DMEM:F12 (1:1) Media for SILAC

Catalog number: 88215

Thermo Scientific™  Related applications: Protein Mass Spectrometry Analysis

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Thermo Scientific DMEM:F12 (1:1) Media for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).

Features of DMEM:F12 (1:1) Media for SILAC:

Flexible—liquid media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
High quality—media are sterile, endotoxin-free and cell culture-compatible

Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of "heavy" 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

This SILAC media is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): Mass Spectrometer
Product Size: 500 mL
Shipping Condition: Wet Ice
Starting Material: Cells
Workflow Step: Protein Labeling

Contents & storage

Store in refrigerator (2 to 8°C).


Manuals & protocols