pMCS minP-Tluc16-DD Vector for Luciferase Assays

Catalog number: 88232

Thermo Scientific™  Related applications: Protein Assays and Analysis | Protein Expression

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Description

The Thermo Scientific™ pMCS minP-Tluc16-DD Vector is a multiple cloning site (MCS) vector designed to accept a response element sequence lacking promoter activity for study of gene regulation using the intracellular TurboLuc™16 (Tluc16) luciferase reporter.

• Intracellular Tlac16 luciferase gene, optimized for high expression in mammalian systems
• Optimized minimal core promoter (minP) and 5′ UTR region for efficient expression of Tluc16 luciferase
• Multiple cloning sites provide versatility for transfer of regulatory elements from one vector to another
• Dual-destabilization (DD) technology reduces accumulation of Tluc16 luciferase mRNA and protein in cells, enhancing the responsiveness of the assay
• Synthetic polyA terminator and Transcriptional Pause Site (TPS) included upstream of MCS to minimize non-specific transcriptional read-through

Tluc16 luciferase is a 16 kDa, novel, intracellular luciferase derived from the marine copedod Metridia luciferase family. The wild-type luciferase has been modified to reduce its size, increase its brightness, and enable its intracellular expression. The Tluc16 luciferase expression vector also contains dual-destabilization (DD) technology that reduces accumulation of Tluc16 luciferase mRNA and protein in cells, enhancing the responsiveness of the assay. A synthetic polyA terminator and a Transcriptional Pause Site (TPS) are included upstream of the MCS to minimize non-specific transcriptional read-through. The Tluc16 luciferase activity can be measured using the Thermo Scientific TurboLuc™ One-Step Glow Assay Kit.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: minP
Key Function: Reporter Assays
Reporter: TurboLuc™16 Luciferase
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Product Size: 10 µg

Contents & storage

Store in freezer (-5 to -30°C).

Documents

Manuals & protocols