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Pierce™ Protease Inhibitor Tablets, EDTA-free

Catalog number: 88266

Thermo Scientific™  Related applications: Protein Purification & Isolation

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Thermo Scientific Pierce EDTA-Free Protease Inhibitor Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments.

Features of EDTA-Free Protease Inhibitor Tablets:

Economical—More cost effective than other commercially available formulations for equivalent volumes
Easy-to-use—the refrigerator-stable tablets are easily dissolved in buffers and lysis reagents
EDTA-free—formulated without EDTA, a metalloproteinase inhibitor
Compatible—added compatibility with IMAC purification methods and 2D gel electrophoresis

Pierce Protease Inhibitor Tablets protect proteins from proteolytic degradation through inhibition of the serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. The tablets contain AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A. Stable for storage at 4°C for up to 12 months, each large tablet is sufficient for 50 mL of solution, and is suitable for reagent preparation prior to protein extraction from tissue and cultured cells.

Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids. Proteases are regulated and compartmentalized in living cells. Protein extraction from tissues and cultured cells causes their release and ability to degrade other valuable proteins, adversely affecting downstream applications. For this reason, it is common practice to add multiple compounds known to inactivate or inhibit proteases to cell lysis buffers or cell extracts.

To use Pierce Protease Inhibitor Tablets, simply dissolve 1 tablet in 10 or 50 mL (respectively) of buffer or lysate. The protease inhibitor formulation is compatible with most detergent-based cell lysis reagents, including Thermo Scientific Pierce Cell Lysis Solutions. Pierce Protease Inhibitor Tablets do not contain phosphatase inhibitors. If necessary, phosphatase inhibitors, such as Thermo Scientific Pierce Phosphatase Inhibitor Tablets, can be added along with the protease inhibitor tablets.

More Product Data
Complete protein protection from proteases and phosphatases

Related Products
Pierce™ Protease Inhibitor Mini Tablets, EDTA Free
Pierce™ Protease Inhibitor Tablets
Pierce™ Protease Inhibitor Mini Tablets
For Research Use Only. Not for use in diagnostic procedures.


Reagent Type: Protease Inhibitor
Form: Tablets
Product Size: 20 tablets

Contents & storage

Upon receipt store at 4°C.

Note: Tablets may discolor if stored for extended periods at room temperature. The color change does not affect inhibitor activity.


Manuals & protocols

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