An new endotoxin detection kit is now available—Pierce Chromogenic Endotoxin Quant Kit, with higher sensitivity and no interference from β-glucans. To learn more about these new kits, please visit thermofisher.com/endotoxin.
Thermo Scientific™

Pierce™ High Capacity Endotoxin Removal Resin

Catalog number:  88270

Related applications:

Protein Purification & Isolation

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Description

Thermo Scientific Pierce High Capacity Endotoxin Removal Resin binds to reduce endotoxin levels in protein samples by ≥99% in 1 hour while maximizing protein recovery. Use this endotoxin removal resin slurry to pack a custom column that can be re-used up to 10 times with no loss in endotoxin removal efficiency.

Features of High Capacity Endotoxin Removal Resin:

High capacity—bind up to 2,000,000 EU/mL to eliminate >99% of endotoxins
Durable—re-use resin up to 10 times with no loss in performance
Selective—recover ≥85% of your protein sample
High performance—meets FDA guidelines by reducing final EU concentration to <5 EU/mL
Economical—large-volume discounts available

The Pierce High Capacity Endotoxin Removal Resin combines porous cellulose beads and an FDA-approved food preservative, poly(ε-lysine), as an affinity ligand to selectively bind endotoxins. Endotoxin levels in protein samples are reduced by ≥99% in as little as 1 hour using our spin cup format, and protein recovery is ≥85%. The modified polylysine affinity ligand eliminates the toxicity concerns associated with alternative technologies using polymixin B ligands and sodium deoxycholate buffers.

Requires:
Endotoxin-free water
Applications:

Eliminate endotoxins from protein or antibody samples prior to cell transfection or animal injection

The presence of endotoxins in biologically derived products prepared for research, vaccination, or therapeutic use is a major concern due to the diverse and potentially harmful biological response to these molecules. Endotoxin contamination in proteins purified from bacterial cultures is due to residual lipopolysaccharides (LPS) derived from the outer cell membrane of gram-negative bacteria such as E. coli. Endotoxin-contaminated protein samples transfected into cells or injected into an animal host can initiate a strong immune response resulting in a Systemic Inflammatory Response Syndrome (SIRS) and/or sepsis. Traditional endotoxin removal resins that use polymixin B and sodium deoxycholate to clear endotoxins from a sample also have the negative side effects of neurotoxicity and acute renal tubular necrosis.

Pierce High Capacity Endotoxin Removal Resin is a porous cellulose bead that has been surface-modified with covalently attached polylysine chains that have a high affinity for LPS but lacks the toxicity of other endotoxin-binding molecules. With a 2,000,000-EU/mL binding capacity, endotoxin levels can be reduced by 99% in samples containing initial endotoxin levels of 10,000 EU/mL. Typical protein samples processed with the High Capacity Endotoxin Removal Resin have a final endotoxin concentration below 5 EU/mL. This resin is offered in a slurry format for custom packing of endotoxin removal columns for gravity flow or flow rates ranging from 10-15 mL/hour.

One endotoxin unit/mL (EU/mL) equals approximately 0.1ng endotoxin/mL of solution.

More Product Data
Removing endotoxins using a spin-column format
Eliminate endotoxins from protein and antibody samples

Related Products
Pierce™ High Capacity Endotoxin Removal Spin Columns, 0.25 mL
Thermo Scientific Pierce High Capacity Endotoxin Removal Resin eliminates &gt;99% of endotoxins from various protein samples

Thermo Scientific Pierce High Capacity Endotoxin Removal Resin eliminates &gt;99% of endotoxins from various protein samples

1. Centrifuge at 500Xg for 1 minute to remove the storage buffer.<br/> 2. Regenerate with either 0.2N NaOH (overnight) or 0.2N NaOH in 95% ethanol for 1-2 hours.<br/> 3. Wash with 2M NaCl followed by ET-free water.<br/> 4. Equilibrate with ET-free buffer, pH 6-8. Repeat three times.<br/> 5. Add sample and incubate at 4-22&#176;C with gentle end-over-end mixing for 1 hour.<br/> 6. Centrifuge at 500 X g for 1 minute to collect the sample.

1. Centrifuge at 500Xg for 1 minute to remove the storage buffer.<br/> 2. Regenerate with either 0.2N NaOH (overnight) or 0.2N NaOH in 95% ethanol for 1-2 hours.<br/> 3. Wash with 2M NaCl followed by ET-free water.<br/> 4. Equilibrate with ET-free buffer, pH 6-8. Repeat three times.<br/> 5. Add sample and incubate at 4-22&#176;C with gentle end-over-end mixing for 1 hour.<br/> 6. Centrifuge at 500 X g for 1 minute to collect the sample.

Chemical structure

Chemical structure

Specifications

Column Type: Proprietary Resin, Affinity
Product Line: Pierce™
Purification Target: Endotoxin Removal
Format: Bottle(s)
Stationary Phase: Proprietary Ligand
Quantity: 10mL

Contents & storage

Upon receipt store at 4°C.