Thermo Scientific Pierce High Capacity Endotoxin Removal Resin eliminates >99% of endotoxins from various protein samples
1. Centrifuge at 500Xg for 1 minute to remove the storage buffer.<br/> 2. Regenerate with either 0.2N NaOH (overnight) or 0.2N NaOH in 95% ethanol for 1-2 hours.<br/> 3. Wash with 2M NaCl followed by ET-free water.<br/> 4. Equilibrate with ET-free buffer, pH 6-8. Repeat three times.<br/> 5. Add sample and incubate at 4-22°C with gentle end-over-end mixing for 1 hour.<br/> 6. Centrifuge at 500 X g for 1 minute to collect the sample.
|Column Type:||Proprietary Resin, Affinity|
|Purification Target:||Endotoxin Removal|
|Stationary Phase:||Proprietary Ligand|