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View additional product information for Pierce™ LAL Chromogenic Endotoxin Quantitation Kit - FAQs (88282)
7 product FAQs found
Any endotoxin-free (EF) 96-well plate can be used with the Pierce LAL Chromogenic Endotoxin Quantitation Kit. Please check the below EF products:
- EF microplates (https://www.fishersci.com/shop/products/endotoxin-free-96wl-mcro-50-cs/NC9217517)
- Thermo Scientific Snap Cap Low Retention Microcentrifuge Tubes (https://www.thermofisher.com/order/catalog/product/3453)
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
The Pierce LAL Chromogenic Endotoxin Quantitation Kit is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended at 4 degrees C. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
It is found on the Certificate of Analysis for each lot of Endotoxin included in an LAL test kit.
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The kit reagents are “matched” to comply with Food and Drug Administration (FDA) requirements for endotoxin testing. Each LAL lot is tested for functionality using the United States Reference Standard EC-6. The LAL lot is then matched to a lot of our Control Standard Endotoxin (CSE) by testing in parallel with the Reference Standard Endotoxin (RSE). The RSE/CSE correlation assay determines the potency of each CSE lot when used with each matching LAL lot. To convert the current FDA RSE (EC-6) from EUs into ng, the conversion is 10 EU/ng.
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If you are measuring endotoxin in a complex sample with many components that you suspect may interfere with the assay, you should check for assay inhibition. If the sample is relatively simple such as a purified protein, there should be no need to check for assay inhibition.
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Assay inhibition occurs when substances in the test sample interfere with the LAL reaction. In the chromogenic assay, this inhibition results in a lower final absorbance, indicating lower levels of endotoxin than what may be present in the test sample. Determine the lack of product inhibition for each sample undiluted or at an appropriate dilution. To verify the lack of product inhibition, spike an aliquot or dilution of a test sample with a known amount of endotoxin (e.g. 0.5 EU/mL). Assay the spiked sample and the unspiked samples to determine the respective endotoxin concentrations. The difference between the two calculated endotoxin values should equal the known concentration of the spike ±25%.
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Components of undiluted serum interfere with the LAL assay. Serum samples must be diluted 50- to 100-fold to be compatible. The serum must be completely free of RBCs, and the diluted sample may need to be heat-shocked (70 degrees C for at least 15 minutes).
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.