The Thermo Scientific Pierce LAL Chromogenic Endotoxin Quantitation Kit measures the amount of endotoxin in a protein, peptide or antibody sample using the Limulus
Amebocyte Lysate (LAL) assay.
Features of the LAL Chromogenic Endotoxin Quantitation Kit:
—detect as little as 0.1 EU/mL (approx. 0.01ng endotoxin per mL)
—perform this assay in 30 minutes using a 96-well microplate
O111:B4 standard in each kit enables accurate endotoxin quantitation
—405nm absorbance reading is compatible with common ELISA plate readers
The endotoxin concentration in a sample is measured using the Pierce LAL Chromogenic Endotoxin Quantitation Kit via a chromogenic signal generated in the presence of endotoxins. Samples can be measured on a microplate absorbance reader at 405nm. A standard curve is created using the E. coli
endotoxin standard included with each kit to calculate endotoxin levels as low as 0.1 EU/mL, where one endotoxin unit/mL (EU/mL) equals approximately 0.1ng endotoxin/mL of solution. Protein and antibody samples can be assayed in about 30 minutes. Determining endotoxin levels is important to assess the efficiency of endotoxin removal methods and prevent endotoxic shock, inflammation and/or sepsis in tissue culture cells and animals injected with endotoxin contaminated proteins.Includes:
Kit contains Limulus
Amebocyte Lysate (LAL), E. coli
endotoxin standard, chromogenic substrate and endotoxin-free water to prepare assay reagents.Applications:
Quantitation of endotoxin levels in a protein, peptide or antibody solution
The LAL method for measuring endotoxin is based on the interaction of endotoxins with the proenzyme Factor C found in circulating amebocytes of the horseshoe crab Limulus polyphemus
. The proteolytic activity of this proenzyme is activated in the presence of lipopolysaccharides (endotoxins) derived from the outer cell membrane of gram-negative bacteria such as E. coli
. The Chromogenic Limulus
Amebocyte Lysate assay measures endotoxin levels by measuring the activity of this protease in the presence of a synthetic peptide substrate that releases p-nitroaniline (pNA) after proteolysis, producing a yellow color that can be measured by reading the absorbance at 405nm.
To accurately measure endotoxin levels in a sample, the LAL assay uses an endotoxin standard of known concentration that is derived from E. coli
strain O111:B4. This standard is provided with each kit and is used to create a standard curve. The endotoxin concentration is determined by extrapolating the absorbance of an unknown sample against this standard curve, similar to ELISA or total protein quantitation assays.More Product Data
• Removing endotoxins using a spin-column format
• Eliminate endotoxins from protein and antibody samples
For Research Use Only. Not for use in diagnostic procedures.