Cardiomyocyte Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits

Catalog number: 88288

Thermo Scientific™  Related applications: Primary Cell Culture

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Description

Cardiomyocyte Isolation Enzyme 1 (with papain) is a tissue-specific dissociation reagent for the isolation of primary cardiomyocytes from neonatal heart tissue from mouse/rat and is a component of the Thermo Scientific™ Pierce™ Primary Cardiomyocyte Isolation Kit (Product No. 88281).

Papain has been demonstrated to be more effective and less harsh than other proteases for tissue dissociation. When used with Cardiomyocyte Isolation Enzyme 2 (with thermolysin), it improves digestion efficiency and cell yield of primary cardiomyocytes.

Papain Information:

Source:Papaya latex
Molecular mass: 23kDa
Optimal pH: 6.0-7.0

More Product Data
One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts

Related Products
Pierce™ Primary Cardiomyocyte Isolation Kit
Cardiomyocyte Isolation Enzyme 2 (with thermolysin) (20X) for Pierce™ Primary Cell Isolation Kits
DMEM for Pierce™ Primary Cell Isolation Kits
Hanks' Balanced Salt Solution (HBSS) without Ca2+, Mg2+ for Pierce™ Primary Cell Isolation Kits
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: Cardiomyocytes, Primary Cells
Product Size: 1 vial
Target Species: Mouse, Rat

Contents & storage

Stability: 1 year at -20°C
Storage of Reconstituted Enzyme: -20°C for 6 months and up to two freeze-thaw cycles, or 1 week at 4°C. Enzyme use after prolonged storage may result in poor performance.

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Manuals & protocols

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