Thermo Scientific Pierce Graphite Spin Columns improve phosphopeptide analysis by efficiently binding hydrophilic peptides and efficiently removing urea, salts and other contaminants before mass spectrometry analysis.
Features of Graphite Spin Columns:
• Convenient spin format for parallel processing of multiple samples • High-binding capacity with excellent recovery of up to 100 µg of hydrophilic peptides per column • Porous graphite resin for cleaning up phosphopeptide samples before MS analysis
Pierce Graphite Spin Columns enable fast and efficient capture, concentration, desalting and elution of hydrophilic peptides in less than 10 minutes. These columns are ideal for improving mass spectrometric (MS) analyses of samples from protein digests), strong-cation exchange fractions, and enriched phosphopeptides eluted from titanium dioxide and immobilized metal affinity chromatography (IMAC) columns and tips.
The Pierce Graphite Spin Columns improve phosphopeptide analysis by efficiently binding hydrophilic peptides and efficiently removing urea, salts and other contaminants before MS analysis. The C18 resins and C18 tips that are commonly used to desalt peptides are excellent for use with hydrophobic peptides but do not efficiently capture hydrophilic peptides, like phosphopeptides, resulting in enrichment of only hydrophobic fragments. The Pierce Graphite Spin Columns address this issue and are ideal for matrix-assisted laser desorption ionization (MALDI) or nanoelectrospray ionization techniques.
For Research Use Only. Not for use in diagnostic procedures.
Final Product Type
For Use With (Equipment)
Microcentrifuge, Mass Spectrometer
Peptides, Protease-digested protein
Sample Clean Up
Contents & Storage
Store at room temperature.
Improved recovery of representative hydrophilic phosphopeptides using graphite spin columns