Thermo Scientific™

Pierce™ Kinase Enrichment Kit with ADP Probe

Catalog number: 88312
Thermo Scientific™

Pierce™ Kinase Enrichment Kit with ADP Probe

Catalog number: 88312
Catalog Number
88312
also known as 0088-312
Unit Size
16 reactions
Price (USD)
678.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
88312
also known as 0088-312
16 reactions
678.00
Your Price:
***
Product Overview
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This Thermo Scientific Pierce Kinase Enrichment Kit utilizes an ActivX™ ADP Probe to covalently label the active site of ATPases, including chaperones and metabolic enzymes, to enable their selective enrichment using a desthiobiotin tag.

ActivX ADP Probes feature an amine-reactive nucleotide analog and a desthiobiotin (biotin analog) tag that facilitates selective labeling of lysines in the kinase active site and then subsequent enrichment and recovery of labeled protein. These features allow identification and profiling of target enzyme classes across samples or assessment of the specificity and affinity of enzyme inhibitors.

Features of the Pierce Kinase Enrichment Kit:

Specific – label only the conserved active-site lysines of nucleotide-binding proteins
Flexible – use for in vitro labeling of ATPase enzymes derived from cells or tissues
Compatible – use with Western blot or mass spectrometry (MS) workflows

Applications:
• Profile small-molecule binding affinities and active-site inhibition in a dose-dependent manner
• Identify dozens to hundreds of inhibitor targets and off-targets from tissues, cells and subcellular proteomes
• Enrichment of enzymes based on function

Thermo Scientific ActivX Desthiobiotin-ADP is a nucleotide derivative that covalently modifies the active site of enzymes at conserved lysine residues in the nucleotide binding site. The structure of these probes consists of a modified biotin (desthiobiotin) attached to the nucleotide through a labile acyl-phosphate bond. Desthiobiotin is a biotin analog that binds less tightly to biotin-binding proteins resulting in binding that is easily reversed by biotin displacement, low pH or heat denaturation.

Desthiobiotin-ADP probes can be used to selectively enrich, identify and profile target enzyme classes or assess the specificity of enzyme inhibitors. Because many ATPases and other nucleotide-binding proteins bind nucleotides or inhibitors even when they are enzymatically inactive, the desthiobiotin probes allow profiling of both inactive and active enzymes in a complex sample. Preincubation of samples with small-molecule inhibitors that compete for active sites can be used to determine inhibitor binding affinity. Active-site nucleotide probes also can be used to identify inhibitor off-targets.

These products are subject to a limited use label license.

Related Products
Pierce™ Kinase Enrichment Kit with ATP Probe
ActivX™ Desthiobiotin-ATP Probe
ActivX™ Desthiobiotin-ADP Probe
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label Type
Biotin & Other Haptens
Product Line
ActivX™, Pierce™
Technique
Pull-Down
Product Type
Kinase Enrichment Kit
Quantity
16 rxn kit
Assay
Protein Quantitation Assay
Detection Method
Chemiluminescent, Mass Spectrometry, Western Blot
Substrate Properties
Chemical Substrate
Substrate Type
Kinase Substrate
Target Enzyme
Kinases (Multiple), Phosphodiesterases, ATPases, GTPases, Phosphatases
Format
Tube(s), Spin Column

Contents & Storage

ActivX Desthiobiotin-ADP Probe is shipped separately with dry ice and stored at -80°C upon receipt. All other kit components are shipped at ambient temperature and stored at 4°C upon receipt.

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