1-Step Heavy Protein IVT Kit

Catalog number: 88330

Thermo Scientific™  Related applications: Protein Expression | Protein Mass Spectrometry Analysis

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Thermo Scientific 1-Step Heavy Protein in vitro Protein Expression Kits enable rapid cell-free expression of recombinant proteins containing stable isotope-labeled (i.e., heavy) amino acids.

Features of the 1-Step Heavy Protein IVT Kit:

Efficient—express heavy proteins with 90 to 95% stable isotope incorporation
Functional—uses the human translational machinery to express more biologically active proteins than other IVT systems
Flexible—express light proteins or heavy proteins containing 13C615N2 L-lysine and/or 13C615N4 L-arginine
Convenient—perform transcription and translation in a single step
High performance—greater yields compared to rabbit reticulocyte in vitro translation

The 1-Step Heavy Protein IVT Kit uses a unique HeLa cell lysate supplemented with heavy amino acids for in vitro translation (IVT) of proteins with 90 to 95% isotope incorporation efficiency in less than 8 hours. Heavy proteins expressed using this system can be used as mass spectrometry controls for sample prep loss, digestion efficiency determination or as quantification standards.

• Identify ideal peptides for targeted quantitation
• Verify protein digestion efficiency
• Control for protein sample prep variability and affinity enrichment loss

Thermo Scientific 1-Step Heavy Protein IVT Kit contains all of the necessary components to express a heavy protein including HeLa cell lysate, proprietary accessory proteins, reaction mix, heavy amino acids, positive-control GFP DNA and the pT7CFE1-CGST-HA-His cloning vector. The benefits of in vitro expression of heavy proteins over traditional in vivo systems include expression of toxic or insoluble proteins, a more rapid protein synthesis and a more economical use of heavy amino acids compared to stable-isotope labeled cell lines. This small-scale expression method makes it easy to express numerous heavy proteins simultaneously or to express large quantities of a single heavy protein up to 100 µg/mL. Tandem affinity purification of heavy proteins is aided using an expression vector containing multiple affinity tags including GST, HA and 6xHis.

More Product Data
Cell-free protein expression system for generating stable isotope-labeled proteins
For Research Use Only. Not for use in diagnostic procedures.


Product Size: 8 reactions
Final Product: Protein
Workflow Step: Protein Labeling
Detection Method: Mass Spectrometry
Starting Material: DNA
For Use With (Equipment): Mass Spectrometer

Contents & storage

Sufficient For: 8 reactions of 25 µL each • HeLa Lysate, 110 µL
• Accessory Proteins, 25 µL
• Reaction Mix, 40 µL
• 13C6 15N4 L-Arginine, 25 µL
• 13C6 15N2 L-Lysine, 25 µL
• Positive Control DNA: pCFE-GFP, 10 µg
• pT7CFE1-CGST-HA-His, 10 µg
• Nuclease-Free Water 1.5 mL

Upon receipt store kit at -80°C. Product is shipped on dry ice


Manuals & protocols