Dialyzed FBS for SILAC

Catalog number: 88440

Thermo Scientific™  Related applications: Protein Mass Spectrometry Analysis

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Thermo Scientific Dialyzed FBS for SILAC is optimized for use with stable isotope labeling of amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).

Features of Dialyzed FBS for SILAC:

Flexible—liquid media deficient in both L-lysine and L-arginine and powdered media deficient in L-leucine, L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
Compatible—Dialyzed FBS is used to culture a variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, K562 and Jurkat
High quality—media are sterile, endotoxin-free and cell culture-compatible

Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of "heavy" 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins. These SILAC media and dialyzed FBS serum supplements are used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.
For Research Use Only. Not for use in diagnostic procedures.


Product Size: 500 mL
Workflow Step: Protein Labeling
Starting Material: Cells
Shipping Condition: Dry Ice
For Use With (Equipment): Mass Spectrometer

Contents & storage

Store in freezer (-20°C). Do not store in a frost-free freezer.


Manuals & protocols

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