The eBioscience BrdU Staining Kit for Flow Cytometry contains the necessary reagents and buffers for identifying and examining proliferating cells of mammalian species by flow cytometric analysis. Cycling cells are incubated with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newly synthesized genomic DNA during the S-phase of mitosis. Following DNA denaturation, the cells are stained for BrdU incorporation along with any other cell surface and/or intracellular targets of interest.
This kit is optimized to achieve brighter staining compared to traditional protocols.
Bovine, Canine, Cow, Human, Monkey, Mouse, Non-human primate, Rat, Rhesus
Intracellular Staining Followed by Flow Cytometric Analysis