Thermo Scientific™

PVDF Transfer Membrane, 0.2 µm, 26.5 cm x 3.75 m

Catalog number:  88520

Related applications:

Protein Electrophoresis & Western Blotting

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Description

Pierce PVDF Transfer Membrane, 0.2 µm, is made of high-quality polyvinylidene difluoride and provides high binding capacity for proteins and nucleic acids for western, southern, and northern blotting methods. The 0.2-µm pore size makes this membranes ideal for protein analysis of small amounts of proteins (as little as 10 pmoles), amino acid analysis, and transfer of small molecular weight proteins and peptides.

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Features:
Membrane: polyvinylidene difluoride (PVDF)
Binding capacity: goat IgG: 448 µg/cm2; BSA: 340 µg/cm2; insulin: 262 µg/cm2
Re-probe characteristics: yes
Pre-activation: required with 100% alcohol (methanol)
Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, chemiluminescence, and radiolabeling
Durability: compatible with most organic solvents, acids, and mild bases

Specifications

Membrane: PVDF Membrane
Pore Size: 0.2 micron

Contents & storage

Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure.