Pierce™ Phosphatase Inhibitor Mini Tablets

Catalog number: 88667

Thermo Scientific™  Related applications: Protein Purification & Isolation

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Description

Thermo Scientific Pierce Phosphatase Inhibitor Tablets provide complete cell lysate and biological sample protection from dephosphorylation by a broad spectrum of serine and threonine phosphatases.

Features of Phosphatase Inhibitor Tablets:

Economical—more cost effective than other commercially available formulations
Easy-to-use—the refrigerator-stable tablets are easily dissolved in buffers and lysis reagents
Complete—the tablets inhibit acid, alkaline, and protein phosphatases
Compatible—use with homebrew or commercially available cell lysis reagents and Thermo Scientific Protein Assays

Pierce Phosphatase Inhibitor Tablets protect phosphoproteins from serine/threonine phosphatases and tyrosine phosphatases. Each Phosphatase Inhibitor Tablet contains sodium fluoride, sodium orthovanadate, sodium pyrophosphate and beta-glycerophosphate for broad-spectrum inhibition of phosphatase activity. Tablets are stable at 4°C for up to 12 months, and each tablet is sufficient for 10 mL of solution. The Pierce Phosphatase Inhibitor Tablets are suitable for use with any cell or tissue extract to preserve protein phosphorylation for downstream applications.

Protein phosphorylation and dephosphorylation states often function as molecular on/off switches for key regulators in biological pathways within the cell, including signal transduction, cell division and apoptosis. To effectively study phosphorylation signaling events, endogenous phosphatases must be inactivated to prevent their uncontrolled activity upon cell lysis. Pierce Phosphatase Inhibitor Tablets preserve phosphoprotein modifications by significantly reducing endogenous acid, alkaline, and protein phosphatase activity.

Pierce Phosphatase Inhibitor Tablets are compatible with most detergent-based cell lysis reagents, including Thermo Scientific Pierce Cell Lysis Solutions. Where incompatibilities arise, the inhibitor components can be removed by dialysis or desalting before performing these procedures.

To use Pierce Phosphatase Inhibitor Tablets, simply remove 1 tablet from the vial and dissolve in 10 mL of buffer or lysate. The phosphatase inhibitor formulation is compatible with most detergent-based cell lysis reagents, including Thermo Scientific Pierce Cell Lysis Solutions. The Pierce Phosphatase Inhibitor Tablets do not contain protease inhibitors. If necessary, protease inhibitors, such as Thermo Scientific Pierce Protease Inhibitor Tablets, can be added along with the phosphatase inhibitor tablet.

More Product Data
Complete protein protection from proteases and phosphatases

Related Products
Halt™ Phosphatase Inhibitor Cocktail
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reagent Type: Phosphatase Inhibitor
Form: Tablets
Product Size: 20 tablets

Contents & storage

Upon receipt store product at 4°C. Product shipped with an ice pack. Note: Tablets may discolor if stored for extended periods at room temperature.

Documents

Manuals & protocols

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